DDX1抗体

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Western blot analysis using DDX1 mouse mAb against Hela (1), MCF-7 (2), A431 (3), PC-3 (4), and Jurkat (5) cell lysate.    

  
  

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     Immunofluorescence analysis of Hela cells using DDX1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.    

  
  

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     Immunofluorescence analysis of Hela cells using DDX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Hela cells using DDX1 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using DDX1 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using DDX1 mouse mAb with DAB staining.    

  
  

The DDX1 antibody is a key tool for studying the DEAD-box helicase 1 (DDX1) protein, a member of the DEAD-box RNA helicase family characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif. DDX1 plays critical roles in RNA metabolism, including transcription, splicing, RNA transport, and viral RNA processing. It is also implicated in maintaining genomic stability and DNA repair. Dysregulation of DDX1 has been linked to cancers, particularly neuroblastoma, where its overexpression correlates with poor prognosis and chemoresistance. Additionally, DDX1 interacts with HIV-1 RNA and facilitates viral replication, making it a potential therapeutic target.

DDX1 antibodies are widely used in research to detect and quantify DDX1 expression in cells or tissues via techniques like Western blotting, immunohistochemistry, immunofluorescence, and co-immunoprecipitation. These antibodies help elucidate DDX1’s subcellular localization, interactions with nucleic acids or proteins, and functional mechanisms in both physiological and pathological contexts. Commercial DDX1 antibodies are typically raised in rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions) to ensure specificity. Validation data, including knockout/knockdown controls, are essential to confirm antibody reliability. Ongoing research leverages DDX1 antibodies to explore its roles in cancer biology, antiviral immunity, and RNA-related diseases, highlighting their importance in translational and basic science.