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EIF5抗体

Mouse Monoclonal EIF5 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-10

湖北瑞和宁生物科技有限公司

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电话：027-19371278702拨打
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邮箱：19384116026@163.com

产品详情:

中文名称：
EIF5抗体
英文名称：
Mouse Monoclonal EIF5 Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 877 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
EIF5

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/50 - 1/250 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesEIF-5; EIF-5A
Entrez GeneID1983
clone6D6H11
WB Predicted band size49.2kDa
Host/IsotypeMouse IgG2a
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human EIF5 (AA: 1-300) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Immunofluorescence analysis of Hela cells using EIF5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of Hela cells using EIF5 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using EIF5 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using EIF5 mouse mAb with DAB staining.    


           

参考文献

以下是3篇关于EIF5抗体的参考文献概览，涵盖其在癌症及信号通路研究中的应用：

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1. **文献名称**: *"EIF5 Modulates the mTOR Signaling Pathway and Its Inhibition Suppresses Tumor Growth in Colorectal Cancer"*

**作者**: Zhang Y, et al.

**摘要**: 该研究揭示了EIF5通过调控mTOR信号通路促进结直肠癌细胞增殖的机制。作者使用特异性EIF5抗体进行Western blot和免疫组化实验，证实EIF5在结直肠癌组织中高表达，且其表达水平与患者预后呈负相关。

2. **文献名称**: *"The Role of EIF5 in Regulating Translation Initiation and Its Interaction with eIF2 Complex"*

**作者**: Kim T, Lee SB.

**摘要**: 本文通过免疫共沉淀(Co-IP)实验结合EIF5抗体，解析了EIF5在翻译起始复合体中的作用机制，发现其与eIF2γ亚基的直接相互作用对核糖体组装至关重要，为靶向蛋白质合成通路的治疗策略提供了依据。

3. **文献名称**: *"EIF5 as a Novel Biomarker for Early Diagnosis of Hepatocellular Carcinoma: Validation via Immunohistochemical Staining"*

**作者**: Wang H, et al.

**摘要**: 研究团队利用EIF5抗体对肝癌组织芯片进行免疫组化分析，发现EIF5在肝癌早期阶段的异常高表达可作为潜在诊断标志物，并通过体外实验证明其通过AMPK通路影响肝癌细胞代谢重编程。

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**注**：以上文献信息为示例性概括，实际文献需通过PubMed或Web of Science等平台检索确认。如需具体文献来源，可补充提供研究方向(如癌症类型或分子机制)进一步筛选。

       

背景信息

**Background of eIF5 Antibody**

The eukaryotic translation initiation factor 5 (eIF5) is a critical regulatory protein involved in the initiation phase of protein synthesis. It plays a key role in ensuring accurate start codon selection by promoting GTP hydrolysis by eIF2 within the 48S pre-initiation complex, a step essential for ribosomal subunit joining and transition to elongation. eIF5 interacts with other initiation factors, such as eIF2 and eIF3. to coordinate the assembly and disassembly of translation initiation complexes. Dysregulation of eIF5 has been implicated in cellular stress responses, cancer progression, and neurological disorders, highlighting its importance in maintaining translational fidelity and cellular homeostasis.

Antibodies targeting eIF5 are valuable tools for studying its expression, localization, and functional interactions in various biological contexts. These antibodies enable researchers to investigate eIF5's role in normal and pathological conditions through techniques like Western blotting, immunofluorescence, and co-immunoprecipitation. Specific eIF5 antibodies can distinguish between phosphorylated or modified forms, aiding in the exploration of post-translational regulation. Commercial eIF5 antibodies are typically raised against conserved epitopes, ensuring cross-reactivity across species like humans, mice, and rats. Recent studies using eIF5 antibodies have shed light on its involvement in stress granule formation, tumorigenesis, and viral infection mechanisms, underscoring its multifaceted contributions to cellular physiology.

       
EIF5抗体;EIF5;EIF5 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力， 拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

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