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TRAF2抗体

Mouse Monoclonal TRAF2 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-10

湖北瑞和宁生物科技有限公司

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产品详情:

中文名称：
TRAF2抗体
英文名称：
Mouse Monoclonal TRAF2 Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 852 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
TRAF2

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesTRAP; TRAP3; MGC:45012
Entrez GeneID7186
clone5C2C3
WB Predicted band size55.8KD
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human TRAF2 (AA: 39-188) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Immunofluorescence analysis of HL-7702 cells using TRAF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Immunofluorescence analysis of MCF-7 cells using TRAF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of Hela cells using TRAF2 mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of HepG2 cells using TRAF2 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using TRAF2 mouse mAb with DAB staining.    


           

参考文献

以下是关于TRAF2抗体的3篇参考文献示例，包含文献名称、作者和简要摘要内容：

1. **文献名称**：**"TRAF2 is essential for JNK but not NF-κB activation and regulates lymphocyte proliferation and apoptosis"**

**作者**：Hsu, H., Shu, H.B., Pan, M.G., Goeddel, D.V.

**摘要**：该研究利用TRAF2抗体验证了TRAF2在JNK信号通路中的关键作用，发现TRAF2缺失会导致JNK激活受阻，但不影响NF-κB通路。实验通过免疫印迹和免疫沉淀揭示了TRAF2在淋巴细胞增殖与凋亡中的调控机制。

2. **文献名称**：**"TRAF2 must bind to cellular inhibitors of apoptosis for TNF-mediated NF-κB activation"**

**作者**：Vince, J.E., Pantaki, D., Feltham, R., et al.

**摘要**：研究通过TRAF2抗体检测蛋白质相互作用，发现TRAF2需与凋亡抑制蛋白(cIAPs)结合才能介导TNF诱导的NF-κB激活。实验表明，TRAF2-cIAP复合物的形成对细胞存活和炎症反应至关重要。

3. **文献名称**：**"TRAF2 regulates the cytoplasmic/nuclear translocation of IκBα/NF-κB in response to oxidative stress"**

**作者**：Sasaki, C.Y., Barberi, T.J., Ghosh, P., et al.

**摘要**：该研究通过TRAF2抗体进行免疫荧光和Western blot分析，发现氧化应激条件下TRAF2通过调控IκBα的亚细胞定位影响NF-κB活性，揭示了TRAF2在氧化应激相关疾病中的潜在作用。

4. **文献名称**：**"The E3 ligase TRAF2 regulates LUBAC activity by modulating HOIP stability"**

**作者**：Zheng, C., Kabaleeswaran, V., Wang, Y., et al.

**摘要**：文章利用TRAF2抗体研究其与线性泛素链组装复合物(LUBAC)的相互作用，发现TRAF2通过调控HOIP蛋白稳定性影响LUBAC活性，进而参与炎症和细胞死亡信号传导。

以上文献均通过TRAF2抗体进行实验验证，涵盖其在信号通路、疾病机制及蛋白质互作中的功能研究。

       

背景信息

TRAF2 (Tumor Necrosis Factor Receptor-Associated Factor 2) is a critical adaptor protein involved in signal transduction pathways mediated by the tumor necrosis factor receptor (TNFR) superfamily and Toll-like receptors (TLRs). It plays a pivotal role in regulating NF-κB and MAPK signaling pathways, which are essential for immune responses, cell survival, apoptosis, and stress responses. TRAF2 interacts with receptors such as TNFR1. TNFR2. and CD40. facilitating the recruitment of downstream signaling components. Its function includes promoting the activation of canonical NF-κB signaling by forming complexes with cIAP1/2 (cellular inhibitor of apoptosis proteins) and stabilizing the kinase RIP1. Conversely, TRAF2 also participates in non-canonical NF-κB signaling by regulating NIK (NF-κB-inducing kinase) degradation. Dysregulation of TRAF2 is linked to inflammatory diseases, autoimmune disorders, and cancer.

TRAF2 antibodies are widely used in research to study its expression, interactions, and signaling mechanisms. These antibodies enable detection of TRAF2 in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Specific antibodies may target distinct epitopes or post-translational modifications, aiding in the investigation of TRAF2’s role in cellular responses. Monoclonal and polyclonal variants are available, with validation across species like human, mouse, and rat. Understanding TRAF2 dynamics through antibody-based assays has advanced insights into its dual roles in pro-survival and pro-apoptotic contexts, as well as its therapeutic potential in modulating inflammatory and oncogenic pathways.

       
TRAF2抗体;TRAF2;TRAF2 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力， 拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

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