ALDH6A1抗体

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Western blot analysis using ALDH6A1 mouse mAb against Jurkat (1), HEK293 (2), Hela (3), MCF-7 (4), and LNcap (5) cell lysate.    

  
  

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     Immunofluorescence analysis of Hela cells using ALDH6A1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.    

  
  

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     Immunofluorescence analysis of Hela cells using ALDH6A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Hela cells using ALDH6A1 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using ALDH6A1 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using ALDH6A1 mouse mAb with DAB staining.    

  
  

The ALDH6A1 antibody is a crucial tool for studying the aldehyde dehydrogenase 6 family member A1 (ALDH6A1) protein, a mitochondrial enzyme encoded by the ALDH6A1 gene. ALDH6A1 catalyzes the NAD-dependent dehydrogenation of malonate semialdehyde and methylmalonate semialdehyde, playing a pivotal role in valine and pyrimidine catabolism. Its function is vital for maintaining metabolic homeostasis, particularly in lipid and amino acid metabolism. Dysregulation or mutations in ALDH6A1 are linked to metabolic disorders, including methylmalonic aciduria and hyperglycinemia, highlighting its clinical relevance.

Researchers employ ALDH6A1 antibodies in various applications, such as Western blotting, immunohistochemistry, and immunofluorescence, to detect protein expression, localization, and abundance in tissues or cell lines. These antibodies are often raised in rabbits or mice using immunogenic peptides derived from conserved regions of the human ALDH6A1 protein. Specificity is validated through knockout controls or siRNA-mediated knockdown to ensure minimal cross-reactivity with other ALDH family members.

Studies utilizing ALDH6A1 antibodies have expanded understanding of its role in mitochondrial dysfunction, cancer metabolism, and rare genetic diseases. For instance, reduced ALDH6A1 expression has been observed in certain cancers, suggesting potential as a biomarker. The antibody's utility in both basic research and diagnostic development underscores its importance in bridging molecular biology with clinical insights.