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RF1抗体新品

Mouse Monoclonal RF1 Antibody

询价 20μl 起订

50μl 起订

100μl 起订

湖北更新日期：2025-05-10

湖北瑞和宁生物科技有限公司

VIP1年

联系人：周经理

电话：027-19371278702拨打

手机：18008634902 拨打

邮箱：19384116026@163.com

产品详情:

中文名称：: RF1抗体

英文名称：: Mouse Monoclonal RF1 Antibody

品牌：: 艾普蒂

产地：: 武汉

保存条件：: Stored at -20°C for 833 year. Avoid repeated freeze / thaw cycles.

产品类别：: 抗体

重组：: 否

应用：: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA

种属反应性：: Human,Mouse,Rat

宿主：: Mouse

偶联物：: 无

靶点：: RF1

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	咨询技术	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	1/200 - 1/400	Human,Mouse,Rat
Elisa	1/10000	Human,Mouse,Rat

产品详情

Aliases	ERF; ETF1; ERF1; TB3-1; D5S1995; SUP45L1
Entrez GeneID	2107
clone	4F9H12
WB Predicted band size	49kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of human RF1 (AA: 288-437) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using mouse mAb against MCF-7 (1), T47D (2), MOLT4 (3), and Raji (4) cell lysate.
Flow cytometric analysis of Hela cells using RF1 mouse mAb (green) and negative control (red).
Flow cytometric analysis of HepG2 cells using RF1 mouse mAb (green) and negative control (red).

参考文献

以下是关于RF1抗体的3篇示例文献(注：以下内容为模拟示例，实际文献需通过数据库检索确认)：

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1. **文献名称**：*Structural analysis of bacterial release factor RF1 using monoclonal antibodies*

**作者**：Smith A, et al.

**摘要**：研究通过制备针对大肠杆菌RF1的单克隆抗体，结合X射线晶体学揭示了RF1与核糖体结合的构象变化，证实其在终止密码子识别中的关键作用。

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2. **文献名称**：*Development of RF1-specific polyclonal antibodies for functional studies in translation termination*

**作者**：Zhang L, et al.

**摘要**：报道了RF1多克隆抗体的开发，并用于免疫共沉淀实验验证RF1与核糖体及mRNA的相互作用，为研究翻译终止机制提供工具。

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3. **文献名称**：*Antibody-based inhibition of RF1 activity in bacterial cell-free systems*

**作者**：Tanaka K, et al.

**摘要**：利用抗RF1抗体特异性抑制其功能，证明RF1缺失导致翻译终止缺陷，为抗生素靶点研究提供新策略。

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**备注**：实际文献需通过PubMed、Google Scholar等平台检索关键词(如“RF1 antibody”“bacterial release factor”)获取。建议结合研究领域(结构生物学、微生物学等)筛选相关论文。

背景信息

The RF1 antibody targets Ribosome Release Factor 1 (RF1), a protein critical for terminating protein synthesis in prokaryotes. RF1 recognizes stop codons UAA and UAG during translation, facilitating the release of nascent polypeptide chains from ribosomes by hydrolyzing the ester bond linking the completed protein to tRNA. Discovered in the 1960s-70s, RF1 works alongside RF2 (which recognizes UAA/UGA) and is structurally distinct from eukaryotic release factors, making it a potential target for antibacterial therapies.

Research on RF1 has advanced understanding of translation termination mechanisms. Structural studies using X-ray crystallography and cryo-EM revealed that RF1 adopts a conserved conformation when bound to ribosomes, employing specific domains to decode stop signals and catalyze peptide release. Its GTPase activity, though less understood, appears to coordinate with ribosomal subunits during termination.

RF1 antibodies are primarily used as research tools to investigate bacterial translation processes, antibiotic resistance mechanisms, and microbial physiology. Recent studies explore RF1 inhibition as an antimicrobial strategy, particularly against multidrug-resistant pathogens. Additionally, these antibodies aid in structural biology studies mapping ribosomal interactions and evolutionary analyses comparing termination factors across species. While primarily confined to basic research, RF1 antibodies hold translational potential for developing novel antibiotics targeting protein synthesis in pathogenic bacteria.

RF1抗体;RF1;RF1 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力，拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期	(2年)
注册资本	50万(元)
员工人数	1-10人
年营业额	￥ 100万以内
经营模式	贸易,工厂,试剂,定制,服务
主营行业	中间体,化学试剂