Rab5a抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using Rab5a mouse mAb against Hela (1) and K562 (2) cell lysate.    

  
  

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     Immunofluorescence analysis of Hela cells using Rab5a mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Hela cells using Rab5a mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using Rab5a mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human duodenum tissues using Rab5a mouse mAb with DAB staining.    

  
  

Rab5a antibody is a crucial tool in studying the molecular mechanisms of intracellular vesicular trafficking. Rab5a, a member of the Rab GTPase family, plays a pivotal role in regulating early endosome fusion, endocytic transport, and membrane dynamics. It functions as a molecular switch, cycling between active GTP-bound and inactive GDP-bound states to coordinate cargo sorting, vesicle formation, and organelle maturation. Dysregulation of Rab5a has been implicated in various pathologies, including cancer metastasis, neurodegenerative disorders, and viral infections.

Rab5a antibodies are specifically designed to detect and quantify Rab5a protein expression in experimental models. They enable researchers to investigate Rab5a's subcellular localization via immunofluorescence, assess protein levels through Western blotting, and explore protein-protein interactions using co-immunoprecipitation. These antibodies are typically developed in host species like rabbits or mice, targeting conserved epitopes in human, mouse, or rat Rab5a isoforms. Validation parameters include specificity checks using knockout cell lines and functional assays confirming antibody performance in relevant applications.

As a marker for early endosomes, Rab5a antibodies are widely used in cell biology research to dissect endocytic pathways, autophagy, and receptor trafficking. Recent studies also utilize them to explore Rab5a's role in immune responses, synaptic vesicle recycling, and extracellular vesicle biogenesis, underscoring their versatility in both basic and translational research.