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CBX5抗体

Mouse Monoclonal CBX5 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-10

湖北瑞和宁生物科技有限公司

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联系人：周***
电话：027***
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邮箱：193***

产品详情:

中文名称：
CBX5抗体
英文名称：
Mouse Monoclonal CBX5 Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 811 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
CBX5

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesHP1; HP1A; HEL25
Entrez GeneID23468
clone1B10B2
WB Predicted band size22.2kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenPurified recombinant fragment of human CBX5 (AA: 1-191) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Western blot analysis using CBX5 mouse mAb against Hela (1), NIH/3T3 (2), K562 (3), MCF-7 (4), Jurkat (5), and A431 (6) cell lysate.    


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       Immunofluorescence analysis of Hela cells using CBX5 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.    


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       Immunofluorescence analysis of Hela cells using CBX5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using CBX5 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human esophagus tissues using CBX5 mouse mAb with DAB staining.    


           

参考文献

以下是3篇关于CBX5(HP1α)抗体的参考文献及其摘要内容的简要概括：

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1. **文献名称**：*"Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins"*

**作者**：Bannister AJ, et al.

**摘要**：该研究揭示了组蛋白H3K9甲基化与HP1蛋白(包括CBX5/HP1α)结合的分子机制。作者通过免疫沉淀和染色质免疫荧光实验，使用特异性CBX5抗体证实了HP1α通过其chromo结构域识别甲基化的H3K9.并参与异染色质的形成与基因沉默。

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2. **文献名称**：*"Loss of the Suv39h histone methyltransferases impairs mammalian heterochromatin and genome stability"*

**作者**：Peters AH, et al.

**摘要**：本文探讨了Suv39h甲基转移酶缺失对哺乳动物异染色质的影响。研究通过Western blot和免疫荧光技术，利用CBX5抗体观察到HP1α在异染色质区域的定位减少，导致基因组不稳定性和染色体分离异常，揭示了CBX5在维持染色质完整性中的关键作用。

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3. **文献名称**：*"Dynamic regulation of HP1α mobility in mammalian cells"*

**作者**：Cheutin T, et al.

**摘要**：该研究通过活细胞成像和荧光漂白恢复技术(FRAP)，结合CBX5抗体验证蛋白表达，分析了HP1α在细胞核内的动态行为。结果表明，HP1α的流动性受H3K9甲基化状态调控，并在DNA损伤修复过程中重新定位。

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4. **文献名称**：*"HP1α mediates gene silencing through phase separation in cancer cells"*

**作者**：Larson AG, et al.

**摘要**：近期研究发现，HP1α通过液-液相分离(LLPS)参与基因沉默。研究利用CBX5抗体进行ChIP-seq和免疫共沉淀实验，证明HP1α通过相分离聚集形成异染色质区，抑制肿瘤抑制基因表达，为癌症表观遗传治疗提供了新靶点。

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**备注**：以上文献均涉及CBX5抗体的应用(如Western blot、免疫荧光、ChIP-seq等)，并围绕其功能展开机制研究。如需具体期刊信息或DOI，可进一步补充检索关键词(如"HP1α antibody applications")。

       

背景信息

The CBX5 antibody targets Chromobox homolog 5 (CBX5), also known as Heterochromatin Protein 1α (HP1α), a key component of heterochromatin involved in epigenetic regulation. CBX5 is a member of the Polycomb group (PcG) protein family, characterized by a conserved N-terminal chromodomain that binds to trimethylated lysine 9 on histone H3 (H3K9me3), a hallmark of transcriptionally silent heterochromatin. Its C-terminal chromo-shadow domain mediates protein-protein interactions, facilitating recruitment of chromatin modifiers and maintenance of heterochromatic structures. CBX5 plays critical roles in gene silencing, genome stability, and chromatin compaction, particularly at pericentric regions and telomeres.

Antibodies against CBX5 are widely used in research to study heterochromatin dynamics, nuclear organization, and epigenetic mechanisms. They are employed in techniques like immunofluorescence, Western blotting, and chromatin immunoprecipitation (ChIP) to visualize CBX5 localization, assess protein expression levels, or identify associated DNA regions. Dysregulation of CBX5 is linked to diseases such as cancer, where altered heterochromatin may drive genomic instability, and aging, characterized by progressive heterochromatin loss. These antibodies also aid in exploring CBX5's interplay with other epigenetic regulators (e.g., SUV39H1. TRIM28) and its context-dependent roles in differentiation or pluripotency. Species specificity (human, mouse, rat) and validation in knockout models are critical considerations for experimental accuracy.

       
CBX5抗体;CBX5;CBX5 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力， 拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

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