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PRDM5抗体

Mouse Monoclonal PRDM5 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-10

湖北瑞和宁生物科技有限公司

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产品详情:

中文名称：
PRDM5抗体
英文名称：
Mouse Monoclonal PRDM5 Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 804 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
PRDM5

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC咨询技术 Human,Mouse,Rat
ICC1/100 - 1/500 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesBCS2; PFM2
Entrez GeneID11107
clone7D4C12
WB Predicted band size73kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenPurified recombinant fragment of human PRDM5 (AA: 1-100) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Western blot analysis using PRDM5 mouse mAb against HL-60 (1), NIH/3T3 (2), SW480 (3), and HEK293 (4) cell lysate.    


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       Immunofluorescence analysis of MCF-7 cells using PRDM5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of HeLa cells using PRDM5 mouse mAb (green) and negative control (red).    


           

参考文献

以下是关于PRDM5抗体的3篇参考文献示例(信息基于公开文献总结，非真实引用)：

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1. **文献名称**：*PRDM5 regulates chromatin structure through histone methyltransferase activity in cancer cells*

**作者**：Smith A, et al.

**摘要**：研究报道了PRDM5在肿瘤中通过H3K9me3修饰抑制基因转录，利用特异性PRDM5抗体进行ChIP-seq分析，证实其与染色质调控区域结合，并验证抗体在免疫沉淀和免疫荧光中的可靠性。

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2. **文献名称**：*Loss of PRDM5 promotes epithelial-mesenchymal transition in colorectal cancer*

**作者**：Zhang L, et al.

**摘要**：通过Western blot和免疫组化(使用兔源PRDM5单克隆抗体)，研究发现PRDM5缺失通过激活Wnt通路促进结直肠癌转移，抗体特异性经siRNA敲低实验验证。

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3. **文献名称**：*PRDM5 interacts with SMAD proteins to inhibit TGF-β signaling*

**作者**：Garcia-Rojas M, et al.

**摘要**：研究利用PRDM5抗体(货号：ab12345)进行免疫共沉淀(Co-IP)，发现PRDM5与SMAD4结合并抑制TGF-β信号传导，抗体在HEK293T细胞系中验证了高特异性。

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4. **文献名称**：*PRDM5 antibody validation for chromatin immunoprecipitation in stem cells*

**作者**：Chen H, et al.

**摘要**：系统评估了多种PRDM5抗体在ChIP-seq中的表现，推荐使用克隆号7D2的小鼠单抗，其在小鼠胚胎干细胞中成功富集PRDM5靶基因启动子区域。

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注：以上文献名为示例，实际研究中请通过PubMed等数据库检索真实文献(可尝试关键词：PRDM5 antibody validation, PRDM5 chromatin immunoprecipitation)。

       

背景信息

PRDM5 (PR/SET Domain 5) is a member of the PRDM protein family characterized by a conserved N-terminal PR/SET domain and varying numbers of zinc finger motifs. It functions as a transcriptional regulator involved in chromatin remodeling, cell adhesion, and extracellular matrix (ECM) organization. PRDM5 is implicated in maintaining tissue integrity and suppressing tumorigenesis by modulating gene expression through histone methylation (e.g., H3K9me) or direct DNA binding. Dysregulation of PRDM5 has been linked to cancers (e.g., colorectal, ovarian) and connective tissue disorders.

PRDM5 antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are typically raised against specific epitopes, such as the N-terminal PR/SET domain or C-terminal zinc finger regions, and validated via techniques like Western blotting, immunohistochemistry (IHC), or immunofluorescence (IF). High-quality antibodies enable detection of PRDM5 in human, mouse, or rat tissues, aiding research into its role in development, disease, and ECM dynamics. Commercial PRDM5 antibodies vary in clonality (monoclonal/polyoclonal), host species (rabbit, mouse), and applications (ChIP, flow cytometry). Researchers must verify antibody specificity using knockout controls or peptide-blocking assays, as off-target binding is common due to structural homology within the PRDM family. Reliable PRDM5 antibodies are critical for elucidating its tumor-suppressive mechanisms and potential therapeutic applications.

       
PRDM5抗体;PRDM5;PRDM5 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力， 拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

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