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  • HIST2H4A(20Me)抗体
  • HIST2H4A(20Me)抗体
  • HIST2H4A(20Me)抗体

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HIST2H4A(20Me)抗体

Mouse Monoclonal HIST2H4A(20Me) Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-10

湖北瑞和宁生物科技有限公司

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产品详情:

中文名称：
HIST2H4A(20Me)抗体
英文名称：
Mouse Monoclonal HIST2H4A(20Me) Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 797 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
HIST2H4A(20Me)

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesH4; H4/n; H4F2; H4FN; FO108; HIST2H4
Entrez GeneID8370
clone3E7H2
WB Predicted band size11.4kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenSynthesized peptide of human HIST2H4A ( AA: GGAKRHRK(Me)VLRDNIQ ).
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Western blot analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.    


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       Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.    


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       Immunofluorescence analysis of HeLa cells using HIST2H4A(20Me) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.    


           

参考文献

以下是关于 **HIST2H4A(H4K20me)抗体** 的3篇参考文献示例(注：部分文献为假设性示例，实际引用需核实)：

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1. **文献名称**：*"H4K20 methylation regulates DNA repair and chromatin compaction"*

**作者**：Beck DB et al.

**摘要**：研究揭示了H4K20单甲基化(H4K20me1)在DNA损伤修复中的作用，利用HIST2H4A特异性抗体证明其通过招募53BP1蛋白促进非同源末端连接(NHEJ)修复，并影响染色质结构稳定性。

2. **文献名称**：*"Aberrant H4K20 methylation in cancer: A potential biomarker for tumor progression"*

**作者**：Nielsen ML et al.

**摘要**：通过免疫组化分析多种癌症样本，发现H4K20me2/3水平与肿瘤分化程度负相关，使用HIST2H4A抗体验证其在结直肠癌中的表观遗传失调可能作为预后标志物。

3. **文献名称**：*"Antibody-specific detection of histone H4 variants and post-translational modifications"*

**作者**：Sakaguchi A & Shabanowitz J

**摘要**：开发了一种针对HIST2H4A亚型H4K20me的高特异性抗体，结合质谱技术验证其选择性，并应用于研究细胞周期中H4K20甲基化的动态变化。

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**注**：以上文献为示例，实际研究中需根据具体需求检索PubMed、Web of Science等数据库获取准确信息。H4K20甲基化(尤其是H4K20me3)的经典研究可参考Schotta et al. (2004. *Genes & Development*) 或 Jørgensen et al. (2013. *Nature Cell Biology*)。

       

背景信息

The HIST2H4A(20Me) antibody is a specific immunological tool designed to detect histone H4 protein variants encoded by the HIST2H4A gene, particularly when methylated at the lysine 20 (K20) residue. Histone H4 is a core component of nucleosomes, playing a critical role in chromatin structure and epigenetic regulation. Post-translational modifications (PTMs) such as methylation at H4K20 are essential for diverse cellular processes, including transcriptional regulation, DNA repair, and chromosome condensation. The HIST2H4A gene, part of the histone cluster on chromosome 1. produces one of several H4 isoforms, with subtle functional or regulatory differences potentially influencing cellular dynamics.

Methylation at H4K20 exists in mono- (me1), di- (me2), or tri-methylated (me3) states, each associated with distinct biological contexts. For example, H4K20me1 is linked to active transcription, while H4K20me3 is enriched in heterochromatin and implicated in genomic stability. The HIST2H4A(20Me) antibody is typically used to study the spatial and temporal distribution of this modification, aiding in research on cell cycle regulation, cancer biology (where aberrant H4K20 methylation is observed), and developmental epigenetics. Its specificity makes it valuable in techniques like ChIP-seq, immunofluorescence, or Western blotting to dissect chromatin states or identify dysregulated epigenetic pathways in disease models. Validation of this antibody often includes peptide competition assays to confirm target selectivity.

       
HIST2H4A(20Me)抗体;HIST2H4A(20Me);HIST2H4A(20Me) Antib;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力， 拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

HIST2H4A(20Me)抗体相关厂家报价

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  • 维百奥（北京）生物科技有限公司 VIP
  • 2025-12-29
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