NOS1抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Immunofluorescence analysis of HeLa cells using NOS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of HeLa cells using NOS1 mouse mAb (green) and negative control (red).    

  
  

Neuronal nitric oxide synthase (NOS1), also known as nNOS, is a critical enzyme responsible for synthesizing nitric oxide (NO), a versatile signaling molecule involved in neurotransmission, vascular regulation, and immune responses. Expressed predominantly in the central and peripheral nervous systems, NOS1 plays key roles in synaptic plasticity, neuronal development, and smooth muscle relaxation. Dysregulation of NOS1 has been implicated in neurological disorders (e.g., Alzheimer’s disease, Parkinson’s disease), cardiovascular diseases, and cancer, making it a significant research target.

NOS1 antibodies are essential tools for detecting and characterizing the expression, localization, and function of the NOS1 protein in biological samples. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. They enable researchers to study NOS1's tissue-specific distribution, post-translational modifications, and interactions with other proteins. Additionally, NOS1 antibodies aid in investigating pathological mechanisms, such as oxidative stress and neuroinflammation, by visualizing NOS1 upregulation or aberrant activity in disease models.

Commercial NOS1 antibodies are typically raised against specific epitopes of human or murine NOS1. with validated cross-reactivity in multiple species. Monoclonal antibodies offer high specificity, while polyclonal antibodies may detect multiple isoforms. Quality validation, including knockout (KO) controls and functional assays, ensures reliability. Researchers must select antibodies based on experimental needs (e.g., detecting full-length protein vs. truncated variants) and confirm specificity to avoid off-target effects, particularly given the structural similarities among NOS family members (NOS1. NOS2. NOS3).