ATG16L1抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using ATG16L1 mouse mAb against Hela (1) cell lysate.    

  
  

ATG16L1 (Autophagy-related 16-like 1) is a critical protein in the autophagy pathway, a conserved cellular process responsible for degrading damaged organelles, proteins, and pathogens. It plays a central role in autophagosome formation, particularly in the LC3 lipidation step. Structurally, ATG16L1 interacts with the ATG5-ATG12 complex to form a larger complex essential for membrane elongation during autophagosome biogenesis. Research on ATG16L1 antibodies has gained momentum due to its association with human diseases, notably inflammatory bowel disease (IBD). A single-nucleotide polymorphism (T300A) in ATG16L1 is strongly linked to Crohn’s disease susceptibility, implicating defective autophagy in IBD pathogenesis.

ATG16L1 antibodies are widely used to study autophagy dynamics, protein localization, and expression levels in various models, including cell lines, tissues, and animal studies. These antibodies help detect full-length ATG16L1 (~70 kDa) and its isoforms, though cross-reactivity or post-translational modifications may require validation via knockout controls. Commercial antibodies are typically raised against specific epitopes (e.g., N-terminal or WD40 repeat domains) and validated for applications like Western blot, immunofluorescence, and immunohistochemistry. Challenges include distinguishing between cytosolic and membrane-bound forms or detecting disease-associated variants. Understanding ATG16L1’s role continues to shed light on autophagy-related mechanisms in immunity, cancer, and infection.