RAD18抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Flow cytometric analysis of Hela cells using RAD18 mouse mAb (green) and negative control (red).    

  
  

The RAD18 antibody is a crucial tool for studying the RAD18 protein, a key player in DNA damage response and repair mechanisms. RAD18. an E3 ubiquitin ligase, forms a complex with RAD6 to mediate monoubiquitination of proliferating cell nuclear antigen (PCNA), a process essential for translesion DNA synthesis (TLS) during replication stress. This post-replication repair pathway allows DNA replication to bypass lesions, preventing replication fork collapse and maintaining genome stability. Dysregulation of RAD18 is linked to genomic instability, cancer progression, and chemoresistance.

RAD18 antibodies are widely used in Western blotting, immunofluorescence, and immunohistochemistry to detect RAD18 expression, localization, and interactions in various biological contexts. Researchers utilize these antibodies to investigate RAD18's roles in DNA repair fidelity, cell cycle regulation, and its crosstalk with other repair pathways like homologous recombination. Commercially available RAD18 antibodies are typically validated in knockout cell lines to ensure specificity, with host species including rabbit and mouse. Studies employing RAD18 antibodies have revealed its overexpression in certain cancers, suggesting potential as a therapeutic target. Recent work also explores RAD18's non-canonical functions in mitochondrial DNA maintenance and viral infection responses. Proper antibody selection remains critical due to potential cross-reactivity with structurally similar E3 ligases.