THAP1抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Immunofluorescence analysis of Hela cells using THAP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Jurkat cells using THAP1 mouse mAb (green) and negative control (red).    

  
  

THAP1 (THAP domain-containing protein 1) is a zinc finger transcription factor encoded by the *THAP1* gene, located on human chromosome 8. It regulates gene expression by binding to specific DNA sequences via its THAP domain, a conserved motif unique to this protein family. THAP1 plays a critical role in cell proliferation, apoptosis, and neuronal development. Mutations in *THAP1* are linked to DYT6 dystonia, a hereditary movement disorder characterized by involuntary muscle contractions.

THAP1 antibodies are immunological tools developed to detect and study the expression, localization, and functional interactions of THAP1 in research. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help elucidate THAP1's role in transcriptional regulation, its involvement in neurodevelopmental pathways, and the molecular mechanisms underlying dystonia. Studies using THAP1 antibodies have revealed its interaction with proteins like HCFC1 and its regulation of target genes such as *RRM1*.

Research with THAP1 antibodies also contributes to understanding how pathogenic mutations disrupt THAP1’s DNA-binding or protein-interaction capabilities, leading to cellular dysfunction. Their application extends to modeling disease mechanisms in vitro or in animal studies, aiding therapeutic discovery for dystonia and related neurological disorders. Validation of THAP1 antibodies remains essential to ensure specificity, given the protein’s structural similarities within the THAP family.