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  • PDPK1抗体
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PDPK1抗体

Mouse Monoclonal PDPK1 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-10

湖北瑞和宁生物科技有限公司

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联系人：周经理
电话：027-19371278702拨打
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邮箱：19384116026@163.com

产品详情:

中文名称：
PDPK1抗体
英文名称：
Mouse Monoclonal PDPK1 Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 750 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
PDPK1

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Monkey,Rat
IF咨询技术 Human,Mouse,Monkey,Rat
IHC1/200 - 1/1000 Human,Mouse,Monkey,Rat
ICC1/50 - 1/200 Human,Mouse,Monkey,Rat
FCM1/200 - 1/400 Human,Mouse,Monkey,Rat
Elisa1/10000 Human,Mouse,Monkey,Rat
   

产品详情

AliasesPDK1; PDPK2; PDPK2P; PRO0461
Entrez GeneID5170
clone7E4G11
WB Predicted band size63.2kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Monkey,Rat
ImmunogenPurified recombinant fragment of human PDPK1 (AA: 457-556) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Western blot analysis using PDPK1 mouse mAb against MCF-7 (1), Hela (2), K562 (3), U937 (4), A549 (5), NIH/3T3 (6), Jurkat (7), PC-12 (8), and Cos7 (9) cell lysate.    


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       Immunofluorescence analysis of A549 cells using PDPK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of A549 cells using PDPK1 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using PDPK1 mouse mAb with DAB staining.    


           

参考文献

以下是3条关于PDPK1抗体的虚构参考文献示例(内容为模拟生成，非真实文献)：

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1. **文献名称**: "Characterization of PDPK1-Specific Antibodies for Detecting Phosphorylation in Cancer Cell Lines"

**作者**: Johnson R, et al.

**摘要**: 本研究验证了两种商业化PDPK1抗体(克隆号#A12和#B7)在Western blot和免疫组化中的特异性，发现#B7抗体可特异性识别PDPK1的Ser241自磷酸化位点。研究证明其在乳腺癌和肺癌组织中的差异表达，提示PDPK1活性与肿瘤进展相关。

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2. **文献名称**: "Role of PDPK1 in PI3K/Akt Signaling Revealed by shRNA and Antibody-Based Inhibition"

**作者**: Chen L, et al.

**摘要**: 通过shRNA敲低和特异性PDPK1抗体(货号ABX-1023)的功能阻断实验，证实PDPK1是Akt激活的关键上游激酶。抗体处理显著抑制结肠癌细胞增殖，为靶向PDPK1的癌症治疗提供依据。

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3. **文献名称**: "A Novel Monoclonal Antibody Targeting PDPK1 for Glioblastoma Diagnosis"

**作者**: Müller S, et al.

**摘要**: 开发了一种高亲和力抗人PDPK1单克隆抗体(mAb-PDPK1-8E4)，可在脑胶质瘤患者的组织切片中特异性标记PDPK1高表达区域，与患者生存期负相关，提示其作为预后生物标志物的潜力。

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注：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar等平台检索(关键词：PDPK1 antibody, PDK1. 3-phosphoinositide-dependent protein kinase-1)。

       

背景信息

PDPK1 (3-phosphoinositide-dependent protein kinase 1), also known as PDK1. is a serine/threonine kinase that plays a pivotal role in cell signaling pathways, particularly the PI3K/Akt (phosphatidylinositol 3-kinase/protein kinase B) cascade. It acts as a master regulator by phosphorylating and activating downstream AGC kinase family members, including Akt, PKC, and SGK, which are critical for cell survival, proliferation, and metabolism. Dysregulation of PDPK1 is implicated in various diseases, such as cancer, diabetes, and neurodegenerative disorders, making it a key therapeutic target.

Antibodies against PDPK1 are essential tools for studying its expression, localization, and activation in biological systems. They are widely used in techniques like Western blotting, immunoprecipitation, and immunohistochemistry to assess PDPK1 levels or phosphorylation status in disease models or drug response studies. These antibodies help identify PDPK1's role in oncogenic signaling, metabolic regulation, or resistance mechanisms. Both monoclonal and polyclonal PDPK1 antibodies are available, often validated for specificity using knockout cell lines or phospho-specific epitope recognition. Researchers also utilize them to explore PDPK1 interactions with other signaling molecules or evaluate its potential as a biomarker. The development of selective PDPK1 inhibitors in preclinical studies further underscores the importance of reliable antibodies for target validation and mechanistic research.

       
PDPK1抗体;PDPK1;PDPK1 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力， 拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

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