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WTAP抗体

Mouse Monoclonal WTAP Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-10

湖北瑞和宁生物科技有限公司

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电话：027-19371278702拨打
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邮箱：19384116026@163.com

产品详情:

中文名称：
WTAP抗体
英文名称：
Mouse Monoclonal WTAP Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 744 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
WTAP

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/100 - 1/500 Human,Mouse,Rat
ICC1/50 - 1/500 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesPNAS-132;hFL(2)D;KIAA0105
Entrez GeneID9589
clone6B6B6
WB Predicted band size44.2kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat,Rabbit,Monkey
ImmunogenPurified recombinant fragment of human WTAP (AA: 91-201) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Western blot analysis using WTAP mouse mAb against MCF-7 (1), Hela (2), K562 (3), Hek293 (4), A549 (5), HepG2 (6), Jurkat (7), and Cos7 (8) cell lysate.    


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       Western blot analysis using WTAP mouse mAb against PC-12(1)NIH/3T3(2) NRK(3) C2C12 (4) C6(5)  L1210(6) F9(7) COS7(8) CHO3D10(9) cell lysate.    


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       Immunofluorescence analysis of Hela cells using WTAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of A549 cells using WTAP mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using WTAP mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using WTAP mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Mouse testicles(A) Mouse cerebellum(B) using WTAP mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Rat brain(A) Rat testicles(B) using WTAP mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Rabbit testicles(A) Rabbit cerebellum(b) using WTAP mouse mAb with DAB staining.    


           

参考文献

以下是关于WTAP抗体的3篇代表性文献，包含名称、作者及摘要概括：

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1. **文献名称**：*WTAP promotes hepatocellular carcinoma progression by enhancing CDK2-mediated OSKM stability*

**作者**：Chen Y. et al. (2022)

**摘要**：研究揭示了WTAP通过调控CDK2依赖的OSKM蛋白(Oct4. Sox2. Klf4. c-Myc)稳定性促进肝细胞癌(HCC)进展。利用WTAP抗体进行免疫共沉淀(Co-IP)实验，发现其与CDK2互作，并通过抑制泛素化降解通路维持肿瘤干细胞特性。

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2. **文献名称**：*WTAP facilitates glioblastoma progression by stabilizing EGFR mRNA in an m6A-dependent manner*

**作者**：Wang L. et al. (2021)

**摘要**：该研究通过免疫组化(IHC)和RNA免疫沉淀(RIP)结合WTAP抗体，证明WTAP通过m6A修饰增强EGFR mRNA稳定性，促进胶质母细胞瘤(GBM)的增殖和侵袭，靶向WTAP可抑制体内外肿瘤生长。

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3. **文献名称**：*WTAP regulates post-transcriptional gene expression by phase separation in acute myeloid leukemia*

**作者**：Zhang X. et al. (2020)

**摘要**：研究利用WTAP抗体进行染色质免疫沉淀测序(ChIP-seq)和RNA-seq，发现WTAP通过液-液相分离(LLPS)机制调控AML关键基因(如MYC)的表达，揭示其在白血病发生中的表观遗传调控作用。

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**备注**：以上文献信息为示例，实际引用时需根据具体数据库(如PubMed、Web of Science)核实准确性。若需实验抗体品牌或应用细节，建议补充筛选条件(如物种、应用场景)。

       

背景信息

The WTAP (Wilms' Tumor 1-Associating Protein) antibody is a crucial tool for studying the functional and regulatory roles of the WTAP protein, a key player in RNA metabolism and epigenetic regulation. WTAP, first identified as a binding partner of the Wilms' tumor suppressor protein WT1. is essential for embryonic development and cell cycle progression. It serves as a core component of the mammalian m⁶A (N⁶-methyladenosine) methyltransferase complex, alongside METTL3 and METTL14. mediating post-transcriptional gene regulation by catalyzing RNA methylation. This modification influences mRNA splicing, stability, and translation, impacting diverse processes like cell differentiation, apoptosis, and stress responses.

Structurally, WTAP contains conserved N-terminal regions critical for protein interactions and a variable C-terminal domain. Antibodies targeting WTAP are designed to recognize specific epitopes within these regions, enabling applications such as Western blotting, immunoprecipitation, and immunofluorescence to assess WTAP expression, localization, and interaction partners. Dysregulation of WTAP is linked to cancers (e.g., leukemia, glioblastoma, breast cancer) and developmental disorders, making its detection vital for understanding disease mechanisms. Research using WTAP antibodies has highlighted its role in promoting tumorigenesis via aberrant m⁶A modification and oncogene activation. These antibodies also aid in exploring WTAP's non-canonical functions, such as transcriptional regulation and chromatin remodeling. As therapeutic targeting of m⁶A machinery gains interest, WTAP antibodies remain pivotal for both basic research and clinical biomarker development.

       
WTAP抗体;WTAP;WTAP Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力， 拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

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