HSF1抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Immunofluorescence analysis of A431 cells using HSF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using HSF1 mouse mAb with DAB staining.    

  
  

The heat shock factor 1 (HSF1) antibody is a critical tool in studying the molecular mechanisms of cellular stress responses. HSF1. a transcription factor, is the master regulator of heat shock proteins (HSPs), which are molecular chaperones essential for protein folding, particularly under stress conditions such as heat, oxidative stress, or proteotoxic insults. HSF1 antibodies are widely used to detect and quantify HSF1 expression, localization, and activation status in various experimental models, including Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP).

HSF1 exists in a monomeric, inactive state under normal conditions but forms trimers upon stress, translocating to the nucleus to bind heat shock response elements (HSEs) in target gene promoters. Post-translational modifications (e.g., phosphorylation, acetylation) regulate its activity, making phospho-specific HSF1 antibodies valuable for studying activation dynamics. Dysregulation of HSF1 is implicated in cancer, neurodegenerative diseases, and aging, driving interest in therapeutic targeting.

Available as monoclonal or polyclonal versions, HSF1 antibodies vary in specificity and applications. Validation via knockout cells or siRNA knockdown is crucial to confirm target specificity. Researchers also utilize these antibodies to explore HSF1’s non-canonical roles in metabolism, development, and immune responses, highlighting its broad biological significance beyond heat shock regulation.