SHC1抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using SHC1 mouse mAb against NIH/3T3 cell lysate.    

  
  

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     Immunofluorescence analysis of A431 cells using SHC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of NIH/3T3 cells using SHC1 mouse mAb (green) and negative control (red).    

  
  

**Background of SHC1 Antibody**

SHC1 (Src homology 2 domain-containing transforming protein 1) is a key adaptor protein involved in intracellular signaling pathways, particularly those mediated by receptor tyrosine kinases (RTKs). It contains multiple functional domains, including a phosphotyrosine-binding (PTB) domain, an SH2 domain, and a CH1 region, which enable it to interact with phosphorylated tyrosine residues on activated receptors and recruit downstream effectors like Grb2-SOS complexes. This facilitates activation of the Ras-MAPK pathway, regulating cell proliferation, differentiation, and survival. Dysregulation of SHC1 has been implicated in cancer, metabolic disorders, and neurodegenerative diseases.

SHC1 antibodies are essential tools for studying its expression, post-translational modifications (e.g., tyrosine phosphorylation at Y317), and subcellular localization. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to investigate SHC1's role in signaling cascades. Given its involvement in oncogenic pathways, SHC1 antibodies also aid in cancer research, particularly in assessing its overexpression or activation in tumors. Additionally, isoform-specific antibodies (e.g., targeting p66Shc) help explore its unique roles in oxidative stress and aging. These antibodies are critical for both basic research and therapeutic target validation.