RHOF抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Flow cytometric analysis of Hela cells using RHOF mouse mAb (green) and negative control (red).    

  
  

The RHOF antibody is a research tool designed to detect and study the Rho-associated protein RHOF (Rho Family GTPase), a member of the Rho GTPase family involved in regulating cytoskeletal dynamics, cell motility, and intracellular signaling. RHOF, also known as Rnd3. belongs to the Rnd subgroup of Rho GTPases, which are atypical due to their constitutive GTP-binding activity and lack of intrinsic GTPase activity. Unlike classical Rho proteins, RHOF does not require GTP loading to activate downstream effectors but instead functions through protein-protein interactions. It plays roles in cell adhesion, migration, and polarity by modulating actin cytoskeleton organization and stress fiber formation.

RHOF antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression, localization, and interactions in cellular models or tissue samples. These antibodies help researchers investigate RHOF's involvement in physiological processes (e.g., neuronal development, immune response) and pathological conditions, including cancer metastasis, neurological disorders, and cardiovascular diseases. Commercially available RHOF antibodies are typically validated for specificity against target epitopes, though cross-reactivity with related Rho family members remains a consideration. Their application advances understanding of RHOF's regulatory mechanisms and potential therapeutic targeting in diseases linked to cytoskeletal dysfunction.