RAB27A抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using RAB27A mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using RAB27A mouse mAb with DAB staining.    

  
  

The RAB27A antibody is a crucial tool in studying the RAB27A protein, a member of the Rab GTPase family involved in regulating intracellular vesicle trafficking and exocytosis. RAB27A plays a pivotal role in controlling secretory pathways, particularly in melanosome transport in melanocytes, lytic granule release in cytotoxic T cells, and platelet dense granule secretion. Mutations in the RAB27A gene are linked to human diseases such as Griscelli syndrome type 2 (characterized by hypopigmentation and immunodeficiency) and Hermansky-Pudlak syndrome type 10.

RAB27A antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These antibodies help elucidate RAB27A's functional mechanisms, including its binding to effector proteins like melanophilin (MLPH) and myosin Va (MYO5A) to mediate vesicle docking. They are also employed in disease models to explore RAB27A's role in cancer metastasis, immune dysregulation, and pigmentary disorders.

Commercial RAB27A antibodies are typically developed in rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions). Validation includes testing in knockout cell lines or tissues to confirm specificity. As dysregulated RAB27A is implicated in pathologies like melanoma and hemophagocytic lymphohistiocytosis (HLH), its antibody serves as a vital reagent for diagnostic and therapeutic research.