RANBP9抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using RANBP9 mouse mAb against Jurkat (1), MOLT4 (2), HEK293 (3), A431 (4), A549 (5), NIH/3T3 (6) cell lysate.    

  
  

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     Western blot analysis using RANBP9 mouse mAb against PC-129(1) Raw264.7(2) NIH/3T3(3)NRK(4)C2C12(5)F9(6)COS-7(7)cell lysate.    

  
  

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     Immunofluorescence analysis of NIH/3T3 cells using RANBP9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.    

  
  

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     Flow cytometric analysis of Jurkat cells using RANBP9 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using RANBP9 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using RANBP9 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Mouse kidney using RANBP9 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Rat kidney using RANBP9 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Rabbit kidney using RANBP9 mouse mAb with DAB staining.    

  
  

The RANBP9 antibody is a crucial tool for studying the RAN Binding Protein 9 (RANBP9), a multifunctional scaffold protein implicated in diverse cellular processes. RANBP9. also known as RanBPM, interacts with numerous signaling molecules, including receptors, kinases, and ubiquitin ligases, facilitating the assembly of protein complexes. It plays roles in signal transduction (e.g., MAPK, integrin, and tyrosine kinase pathways), cell cycle regulation, apoptosis, and neuronal development. Structurally, RANBP9 contains conserved domains like SPRY, LISH, and CTLH, enabling its scaffolding function. Dysregulation of RANBP9 is linked to neurodegenerative diseases, such as Alzheimer’s, where it promotes amyloid precursor protein (APP) processing, and cancer, where it exhibits dual roles as either a tumor suppressor or metastasis promoter depending on context.

RANBP9 antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and interactions. Their application has advanced research into disease mechanisms, particularly in neuropathology and oncology. For example, studies utilizing these antibodies have revealed RANBP9’s involvement in modulating LRRK2 activity in Parkinson’s disease and its interaction with c-Met in cancer metastasis. As a research reagent, RANBP9 antibodies remain vital for unraveling the protein’s complex roles in cellular homeostasis and pathology, offering potential insights for therapeutic targeting.