RPTOR抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Immunofluorescence analysis of HepG2 cells using RPTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Immunohistochemical analysis of paraffin-embedded human muscle tissues using RPTOR mouse mAb with DAB staining.    

  
  

The RPTOR (Regulatory-Associated Protein of mTOR) antibody is a crucial tool in studying the mechanistic target of rapamycin (mTOR) signaling pathway, which regulates cell growth, proliferation, and metabolism. RPTOR, a core component of mTOR complex 1 (mTORC1), acts as a scaffold protein that recruits substrates and facilitates mTORC1 activation in response to nutrients, growth factors, and cellular energy status. Dysregulation of RPTOR-mediated mTORC1 signaling is implicated in cancer, metabolic disorders, and aging.

The RPTOR antibody enables researchers to detect and quantify RPTOR protein expression levels, assess its post-translational modifications (e.g., phosphorylation), and investigate its subcellular localization. It is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Specific RPTOR antibodies are validated for species cross-reactivity (e.g., human, mouse, rat) and tested for specificity using knockout controls to minimize off-target signals.

In research, this antibody aids in exploring mTORC1's role in diseases, drug discovery (e.g., mTOR inhibitors), and mechanistic studies of nutrient-sensing pathways. For example, it helps evaluate RPTOR expression changes under conditions like starvation, growth factor stimulation, or pharmacological inhibition. Its application extends to cancer biology, where mTORC1 hyperactivation is common, and to metabolic studies linking RPTOR to insulin signaling and autophagy regulation. Validation data, including molecular weight confirmation (~150 kDa) and functional assays, are critical for ensuring experimental reliability.