GAB2抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Immunofluorescence analysis of Hela cells using GAB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Hela cells using GAB2 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using GAB2 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using GAB2 mouse mAb with DAB staining.    

  
  

**Background of GAB2 Antibody**

The **GAB2 (Grb2-associated binding protein 2)** antibody is a tool used to detect and study GAB2. an adaptor protein involved in signal transduction pathways regulating cell growth, differentiation, and survival. GAB2 lacks intrinsic enzymatic activity but serves as a scaffolding molecule, facilitating interactions between signaling proteins. It contains multiple domains, including a pleckstrin homology (PH) domain for membrane targeting, phosphorylated tyrosine residues for SH2 domain-mediated interactions, and binding sites for Grb2. a critical linker in receptor tyrosine kinase (RTK) signaling.

GAB2 is notably associated with pathways like MAPK/ERK and PI3K/AKT, which are frequently dysregulated in cancers. Overexpression or mutations in GAB2 have been implicated in malignancies (e.g., leukemia, breast cancer) and neurodegenerative diseases (e.g., Alzheimer’s). Antibodies targeting GAB2 enable researchers to investigate its expression, post-translational modifications (e.g., phosphorylation), and localization in tissues or cell lines via techniques like Western blot, immunohistochemistry, or immunofluorescence.

Monoclonal GAB2 antibodies offer high specificity, while polyclonal versions may detect multiple epitopes, enhancing sensitivity. These reagents are vital for understanding GAB2’s role in disease mechanisms and its potential as a therapeutic target. For instance, GAB2 inhibition is explored in cancers with hyperactive RTK signaling. Additionally, GAB2 antibodies aid in biomarker studies, correlating protein levels with disease progression or treatment response. Ongoing research continues to unravel its complex interactions in health and disease.