CAMK2G抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using CAMK2G mouse mAb against PC-12 (1), Jurkat (2), T47D (3), HepG2 (4) cell lysate.    

  
  

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     Western blot analysis using CAMK2G mouse mAb against PC-12(1)Raw264.7 (2)NIH/3T3(3) NRK (4) C2C12(5)C6(6)F9(7)COS-7(8)CHO3D10(9) cell lysate.    

  
  

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     Flow cytometric analysis of Jurkat cells using CAMK2G mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using CAMK2G mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using CAMK2G mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Mouse cerebellum using CAMK2G mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Rat cerebellum using CAMK2G mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Rabbit spinal cord using CAMK2G mouse mAb with DAB staining.    

  
  

The CAMK2G antibody targets the gamma isoform of calcium/calmodulin-dependent protein kinase II (CAMKII), a multifunctional serine/threonine kinase central to calcium signaling pathways. CAMKII is composed of four isoforms (α, β, γ, δ), with CAMK2G (γ) widely expressed in tissues such as the brain, heart, and skeletal muscle. It plays critical roles in synaptic plasticity, memory formation, cardiac contraction, and cell cycle regulation. Activation occurs via calcium/calmodulin binding, triggering autophosphorylation at Thr287. which sustains kinase activity even after calcium levels decline.

CAMK2G antibodies are essential tools for detecting protein expression, localization, and activation states in research. They are used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specificity is crucial due to high homology among CAMKII isoforms; rigorous validation via knockout controls or siRNA is recommended. Phosphorylation-specific antibodies (e.g., anti-pThr287) help assess activation status in signaling studies.

In neuroscience, CAMK2G antibodies aid in studying synaptic function and neurodegenerative diseases. Cardiovascular research focuses on its role in arrhythmias and hypertrophy. In cancer, CAMK2G is implicated in tumor progression via cell proliferation pathways. Reliable antibodies enable insights into its dual roles in physiological processes and disease mechanisms, highlighting its potential as a therapeutic target.