MEF2A抗体

+ +

•  

   

     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

•  

   

     Immunofluorescence analysis of Hela cells using MEF2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)    

  
  

•  

   

     Flow cytometric analysis of HepG2 cells using MEF2A mouse mAb (green) and negative control (red).    

  
  

**Background of MEF2A Antibody**

The MEF2A (Myocyte Enhancer Factor 2A) antibody is a tool used to detect and study the MEF2A protein, a member of the MEF2 transcription factor family (MEF2A, B, C, D). MEF2A plays critical roles in cellular differentiation, development, and survival, particularly in muscle cells, neurons, and cardiac tissue. It binds to specific DNA sequences via a conserved MADS-box domain and a MEF2-specific motif, regulating genes involved in cell cycle control, apoptosis, and stress responses.

MEF2A is implicated in cardiovascular biology, neurodevelopment, and diseases such as muscular dystrophy, Alzheimer’s, and coronary artery disease. Its activity is tightly regulated by post-translational modifications (e.g., phosphorylation) and interactions with cofactors like histone deacetylases (HDACs).

Antibodies targeting MEF2A are essential for investigating its expression, localization, and function in various tissues. They are widely used in techniques like Western blotting, immunohistochemistry, chromatin immunoprecipitation (ChIP), and immunofluorescence. Validation of MEF2A antibodies includes testing for specificity through knockdown/knockout controls and cross-reactivity checks against other MEF2 isoforms.

Research utilizing MEF2A antibodies has advanced understanding of its role in disease mechanisms and potential therapeutic targeting, making it a vital reagent in molecular and cellular biology studies.