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PTPRC抗体

Mouse Monoclonal PTPRC Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期:2025-05-10

湖北瑞和宁生物科技有限公司

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产品详情:

中文名称:
PTPRC抗体
英文名称:
Mouse Monoclonal PTPRC Antibody
品牌:
艾普蒂
产地:
武汉
保存条件:
Stored at -20°C for 629 year. Avoid repeated freeze / thaw cycles.
产品类别:
抗体
重组:
应用:
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性:
Human,Mouse,Rat
宿主:
Mouse
偶联物:
靶点:
PTPRC

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesLCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Entrez GeneID5788
clone2C10C11
WB Predicted band size147.3kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human PTPRC (AA: 928-989) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Western blot analysis using PTPRC mouse mAb against Hela (1) and A431 (2) cell lysate.    


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       Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using PTPRC mouse mAb with DAB staining.    


           

参考文献

以下是关于PTPRC抗体的3篇参考文献示例(文献信息为模拟概括,实际引用需核实具体来源):

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1. **标题**: *CD45 (PTPRC) Isoforms in T Cell Signaling and Therapeutic Targeting*

**作者**: Hermiston ML et al.

**摘要**: 研究CD45不同剪接异构体在T细胞激活中的调控作用,探讨其抗体在调节免疫反应及治疗自身免疫疾病中的潜在应用。

2. **标题**: *PTPRC Antibody as a Biomarker for Leukemia Subtype Classification*

**作者**: Smith J, Brown K.

**摘要**: 通过PTPRC抗体对白血病细胞表面抗原检测,建立针对不同白血病亚型的诊断方法,验证其在临床分型中的特异性与敏感性。

3. **标题**: *Structural Insights into CD45 Phosphatase Domain via Monoclonal Antibody Binding*

**作者**: Zhang Y et al.

**摘要**: 利用单克隆抗体解析CD45蛋白酪氨酸磷酸酶结构域构象变化,揭示抗体结合对其酶活性的抑制作用,为靶向药物开发提供依据。

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注:以上内容基于PTPRC抗体的典型研究方向概括,实际文献需通过PubMed或学术数据库检索确认。

       

背景信息

The PTPRC antibody targets the protein tyrosine phosphatase receptor type C (PTPRC), commonly known as CD45 or leukocyte common antigen (LCA). This transmembrane glycoprotein is a receptor-type tyrosine phosphatase expressed exclusively on nucleated hematopoietic cells, including T and B lymphocytes, natural killer cells, monocytes, and granulocytes. It plays a crucial role in immune cell signaling by regulating antigen receptor signaling through the dephosphorylation of Src-family kinases, thereby modulating lymphocyte activation, differentiation, and survival.

Structurally, CD45 contains a cytoplasmic phosphatase domain and an extracellular domain with variable isoforms generated by alternative splicing of exons 4-6 (A, B, and C). These isoforms (e.g., CD45RA, CD45RO) serve as markers for distinguishing naive and memory T-cell subsets. The PTPRC gene is located on chromosome 1q31-q32.

PTPRC antibodies are widely used in research and diagnostics to identify immune cell populations via flow cytometry, immunohistochemistry, or Western blot. They are essential in studying immune dysregulation, including autoimmune diseases (e.g., multiple sclerosis), leukemia/lymphoma classification (CD45 absence helps identify immature blasts), and HIV pathogenesis. Additionally, CD45 expression patterns aid in monitoring immune reconstitution post-transplant or during immunotherapy. Its conserved structure across mammals makes it a valuable cross-species marker.

       
PTPRC抗体;PTPRC;PTPRC Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ¥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

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