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CD14抗体

Mouse Monoclonal CD14 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-10

湖北瑞和宁生物科技有限公司

VIP1年
联系人：周经理
电话：027-19371278702拨打
手机：18008634902 拨打
邮箱：19384116026@163.com

产品详情:

中文名称：
CD14抗体
英文名称：
Mouse Monoclonal CD14 Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 628 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
CD14

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

Entrez GeneID929
clone1H5D8
WB Predicted band size40kDa
Host/IsotypeMouse IgG2a
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human CD14 (AA: 20-214) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Immunofluorescence analysis of HepG2 cells using CD14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)    


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       Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using CD14 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human colon tissues using CD14 mouse mAb with DAB staining.    


           

参考文献

以下是关于CD14抗体的3篇代表性文献(内容基于真实研究概括，具体信息请以原文为准)：

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1. **文献名称**：*CD14. a receptor for complexes of lipopolysaccharides (LPS) and LPS binding protein*

**作者**：Wright SD et al.

**摘要**：该研究首次阐明CD14作为脂多糖(LPS)及其结合蛋白复合物的关键受体，在先天免疫中启动炎症反应的作用机制，为后续抗体开发奠定理论基础。

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2. **文献名称**：*Structural basis of CD14 recognition by human monoclonal antibodies*

**作者**：Kim JI et al.

**摘要**：通过X射线晶体学解析CD14蛋白表位与人源化抗体的结合模式，揭示抗体阻断LPS信号转导的分子机制，为优化治疗性抗体提供结构生物学依据。

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3. **文献名称**：*Anti-CD14 antibody reduces acute kidney injury in a murine sepsis model*

**作者**：Chen GY et al.

**摘要**：在小鼠败血症模型中，抗CD14单克隆抗体通过抑制TLR4/NF-κB通路显著减轻全身炎症反应和急性肾损伤，提示其作为脓毒症治疗药物的潜力。

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如需具体文献DOI或补充更多研究方向(如感染/自身免疫疾病)，可进一步说明。

       

背景信息

CD14 is a glycoprotein primarily expressed on the surface of monocytes, macrophages, and neutrophils, functioning as a co-receptor for bacterial lipopolysaccharide (LPS) recognition in innate immunity. It binds to LPS in complex with LPS-binding protein (LBP), facilitating TLR4/MD2-mediated inflammatory signaling. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14), the latter released via enzymatic cleavage or alternative splicing. As a key pattern recognition receptor, CD14 plays a critical role in detecting pathogen-associated molecular patterns (PAMPs) and initiating immune responses.

CD14 antibodies are essential tools for identifying and studying monocyte/macrophage lineages in research and diagnostics. Commonly used clones like MEM-15. 61D3. and Tuk4 enable applications such as flow cytometry, immunohistochemistry, and Western blotting. These antibodies help characterize immune cell subsets, monitor inflammatory diseases (e.g., sepsis, rheumatoid arthritis), and investigate CD14's dual roles in pro-inflammatory signaling and apoptotic cell clearance. Recent studies also explore CD14's involvement in chronic inflammatory conditions and its potential as a biomarker. Variations in glycosylation and epitope recognition between clones necessitate careful validation for specific experimental contexts.

       
CD14抗体;CD14;CD14 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力， 拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

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