DIS3L2抗体 新品

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using DIS3L2 mouse mAb against Hela (1) and HepG2 (2) cell lysate.    

  
  

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     Flow cytometric analysis of Jurkat cells using DIS3L2 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using DIS3L2 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using DIS3L2 mouse mAb with DAB staining.    

  
  

The DIS3L2 antibody is a crucial tool for studying the DIS3 Like 3'-5' Exoribonuclease 2 (DIS3L2), an RNA-processing enzyme involved in cytoplasmic RNA decay. DIS3L2. a member of the RNase II/R family, degrades diverse RNA substrates, including mRNAs, non-coding RNAs, and aberrant transcripts, often in collaboration with the exosome complex or through poly(U)-binding mechanisms. It plays a vital role in maintaining RNA homeostasis, impacting cellular processes like development, stress responses, and cell cycle regulation.

Mutations or dysregulation of DIS3L2 are linked to human diseases. Biallelic mutations cause Perlman syndrome, a congenital overgrowth disorder, while somatic deletions or mutations are observed in myelodysplastic syndromes and cancers. DIS3L2 also mediates the decay of uridylated miRNAs and mRNAs, connecting it to post-transcriptional gene regulation.

The DIS3L2 antibody, typically raised against specific epitopes (e.g., N-terminal or C-terminal regions), enables detection of endogenous DIS3L2 via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Its specificity is validated using knockout controls or siRNA-mediated depletion. Researchers use this antibody to explore DIS3L2's expression patterns, subcellular localization, and interactions with RNA-binding partners, aiding investigations into its role in RNA quality control, disease pathogenesis, and potential therapeutic targeting.