PHC1抗体

The PHC1 (Polyhomeotic Homolog 1) antibody is a tool used to study the PHC1 protein, a core component of the Polycomb Repressive Complex 1 (PRC1) within the Polycomb group (PcG) family. PcG proteins are evolutionarily conserved epigenetic regulators that maintain transcriptional repression of genes, particularly those involved in cell fate determination, differentiation, and development. PHC1. along with other PRC1 subunits (e.g., BMI1. RING1B), facilitates chromatin compaction and monoubiquitination of histone H2A at lysine 119 (H2AK119ub), reinforcing gene silencing. This activity is critical for preserving stem cell pluripotency and ensuring proper embryonic development. Dysregulation of PHC1/PRC1 is linked to cancers, neurological disorders, and developmental anomalies. PHC1 antibodies are widely employed in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate its expression, localization, and interactions in cellular and disease models. They also aid in exploring PRC1's role in epigenetic memory, X-chromosome inactivation, and tumorigenesis. Validating PHC1 antibody specificity is essential due to structural similarities among PcG proteins. Research utilizing these antibodies has advanced understanding of Polycomb-mediated gene regulation and its therapeutic implications.