CAMK4抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using CAMK4 mouse mAb against Jurkat (1), SK-N-SH (2), Raji (3), and HeLa (4) cell lysate.    

  
  

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     Immunofluorescence analysis of HepG2 cells using CAMK4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using CAMK4 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using CAMK4 mouse mAb with DAB staining.    

  
  

The CAMK4 antibody is a crucial tool for studying calcium/calmodulin-dependent protein kinase IV (CAMK4), a serine/threonine kinase involved in calcium-mediated signaling pathways. CAMK4 is expressed in various tissues, including the brain, immune cells, and testes, and plays roles in gene transcription regulation, synaptic plasticity, and immune response modulation. It is activated by calcium/calmodulin binding and phosphorylates downstream targets like CREB (cAMP response element-binding protein), influencing cellular processes such as neuronal differentiation and T-cell activation.

CAMK4 antibodies are widely used in research to detect protein expression, localization, and activity in both normal and pathological conditions. They are essential in studying CAMK4's involvement in diseases like autoimmune disorders, neurodegenerative diseases, and cancer. Common applications include Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Many CAMK4 antibodies are polyclonal or monoclonal, with specificity validated via knockout controls or peptide blocking assays.

Researchers must consider cross-reactivity with related kinases (e.g., CAMK2) and optimize experimental conditions (e.g., fixation methods, buffer compositions) to ensure accuracy. Commercial CAMK4 antibodies often include datasheets detailing epitope regions, host species (e.g., rabbit, mouse), and recommended dilutions. Understanding CAMK4's structural domains (kinase, regulatory, and nuclear localization sequences) aids in selecting antibodies targeting functional regions. Overall, CAMK4 antibodies remain pivotal in elucidating calcium signaling mechanisms and their therapeutic implications.