ENO2抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using ENO2 mouse mAb against Mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate.    

  
  

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     Flow cytometric analysis of HeLa cells using ENO2 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using ENO2 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using ENO2 mouse mAb with DAB staining.    

  
  

The ENO2 antibody targets enolase 2 (ENO2), a glycolytic enzyme encoded by the *ENO2* gene, also known as neuron-specific enolase (NSE). ENO2 catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and is predominantly expressed in neurons, neuroendocrine cells, and certain cancers. Its neuron-specific expression has made it a widely used biomarker for neuronal injury, neuroendocrine tumors, and small cell lung cancer.

ENO2 antibodies are critical tools in research and diagnostics, enabling the detection and quantification of ENO2 protein levels via techniques like immunohistochemistry (IHC), Western blotting, and ELISA. In clinical contexts, elevated serum NSE levels (detected using ENO2 antibodies) correlate with neurological damage (e.g., stroke, traumatic brain injury) and malignancies.

Beyond its metabolic role, ENO2 exhibits "moonlighting" functions, including interactions with plasminogen and modulation of hypoxia-inducible pathways, suggesting involvement in tumor invasiveness and metastasis. Studies also explore ENO2's role in neurodegenerative diseases like Alzheimer’s, where its dysregulation may contribute to pathological processes.

Commercially available ENO2 antibodies vary in specificity (monoclonal vs. polyclonal) and applications, requiring validation for cross-reactivity with other enolase isoforms (e.g., ENO1). Ongoing research aims to refine ENO2-targeted therapies and diagnostics, emphasizing its dual role as a metabolic enzyme and a multifunctional signaling molecule in health and disease.