ARHGDIA抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using ARHGDIA mouse mAb against Jurkat (1), HeLa (2), NIH3T3 (3), C6 (4), K562 (5), and COS7 (6) cell lysate.    

  
  

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     Immunofluorescence analysis of HepG2 cells using ARHGDIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.    

  
  

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     Flow cytometric analysis of HeLa cells using ARHGDIA mouse mAb (green) and negative control (red).    

  
  

**Background of ARHGDIA Antibody**

The ARHGDIA (Rho GDP-Dissociation Inhibitor Alpha) antibody is a tool used to detect and study the ARHGDIA protein, a key regulator of Rho GTPases—a family of signaling molecules involved in cytoskeletal dynamics, cell migration, proliferation, and gene expression. ARHGDIA, also known as RhoGDIα, functions by binding to inactive GDP-bound Rho proteins (e.g., RhoA, Rac1. Cdc42), sequestering them in the cytoplasm and preventing their activation by guanine nucleotide exchange factors (GEFs). This regulation is critical for maintaining cellular homeostasis and spatially controlled signaling.

ARHGDIA is ubiquitously expressed and plays roles in diverse physiological and pathological processes, including cancer metastasis, neuronal development, and immune responses. Dysregulation of ARHGDIA has been linked to tumor progression, neurodegenerative disorders, and inflammatory diseases, making it a subject of interest in biomedical research.

The ARHGDIA antibody is widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to analyze protein expression, localization, and interactions. It aids in exploring how ARHGDIA modulates Rho GTPase activity, impacts cellular behaviors, and contributes to disease mechanisms. Researchers also utilize this antibody to validate ARHGDIA knockout or knockdown models and assess its potential as a therapeutic target. Commercial ARHGDIA antibodies are typically validated for specificity and sensitivity across human, mouse, and rat samples, ensuring broad applicability in experimental systems.