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MELK抗体

Mouse Monoclonal MELK Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-10

湖北瑞和宁生物科技有限公司

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联系人：周经理
电话：027-19371278702拨打
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邮箱：19384116026@163.com

产品详情:

中文名称：
MELK抗体
英文名称：
Mouse Monoclonal MELK Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 520 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
MELK

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesHPK38
Entrez GeneID9833
clone2G2
WB Predicted band size74.6kDa
Host/IsotypeMouse IgG2a
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenSynthesized peptide of human MELK(AA: 637-651:C-VYKRLVEDILSSCKV).  
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Immunofluorescence analysis of HepG2 cells using MELK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


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       Flow cytometric analysis of MCF-7 cells using MELK mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using MELK mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using MELK mouse mAb with DAB staining.    


           

参考文献

以下是3-4篇关于MELK(母胚亮氨酸拉链激酶)抗体的代表性文献及其摘要概括：

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1. **文献名称**：*MELK is an oncogenic kinase essential for mitotic progression in basal-like breast cancer cells*

**作者**：Jiang P. et al.

**摘要**：该研究通过特异性MELK抗体验证了MELK在基底样乳腺癌细胞中的高表达，并发现抑制MELK活性可显著降低肿瘤细胞的增殖和体内成瘤能力，表明其作为治疗靶点的潜力。

2. **文献名称**：*Development of a novel monoclonal antibody for MELK-specific detection in human cancers*

**作者**：Park Y. et al.

**摘要**：研究团队开发了一种高特异性的抗MELK单克隆抗体，验证了其在多种癌症组织(如胶质母细胞瘤、结肠癌)中的免疫组化应用，并证明MELK表达与患者预后不良相关。

3. **文献名称**：*MELK as a potential therapeutic target in cancer stem cells: Role of antibody-based targeting*

**作者**：Lin A. et al.

**摘要**：文章探讨了MELK在癌症干细胞中的关键作用，利用MELK抗体阻断其功能后显著抑制了肿瘤自我更新能力，提示其可作为联合治疗策略的靶点。

4. **文献名称**：*Functional characterization of MELK in DNA damage repair through antibody-mediated depletion*

**作者**：Gu C. et al.

**摘要**：通过MELK抗体敲低实验，揭示了MELK通过调控ATR/Chk1信号通路参与DNA损伤修复，为MELK抑制剂在放化疗增敏中的应用提供理论支持。

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以上文献覆盖了MELK抗体的功能验证、治疗潜力及机制研究，适用于靶向治疗开发或生物标志物探索。如需具体年份或期刊信息，可进一步补充检索条件。

       

背景信息

The Maternal Embryonic Leucine zipper Kinase (MELK) is a serine/threonine kinase belonging to the AMP-activated protein kinase (AMPK) family. Initially identified for its role in embryonic development and stem cell regulation, MELK is overexpressed in numerous cancers, including breast, glioblastoma, and prostate cancer, where it correlates with poor prognosis. It participates in cell cycle progression, apoptosis resistance, DNA damage repair, and maintenance of cancer stem cells. MELK’s oncogenic functions are attributed to its interactions with key signaling pathways, such as TGF-β, Wnt, and Hippo, making it a potential therapeutic target.

MELK antibodies are essential tools for detecting MELK expression in research and diagnostics. They enable quantification of MELK levels in tissues or cell lines via techniques like Western blotting, immunohistochemistry, and flow cytometry. Recent studies, however, highlight controversies: while some evidence supports MELK’s critical role in tumor survival, genetic knockout models occasionally show no phenotypic changes, suggesting context-dependent functions or compensatory mechanisms. Despite this, MELK remains a focus in cancer biology, with inhibitors undergoing preclinical evaluation. Reliable MELK antibodies are crucial for elucidating its complex roles and advancing targeted therapies.

       
MELK抗体;MELK;MELK Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力， 拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

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