mouse Lplunc1抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Immunofluorescence analysis of HeLa cells using Lplunc1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    

  
  

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     Flow cytometric analysis of MCF-7 cells using Lplunc1 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using Lplunc1 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human muscle tissues using Lplunc1 mouse mAb with DAB staining.    

  
  

The mouse Lplunc1 (Palate, Lung, and Nasal Epithelium Clone 1) antibody is a tool used to detect and study the Lplunc1 protein, a member of the Bactericidal/Permeability-Increasing (BPI) protein family. Lplunc1. also known as BPIFA1 or SPLUNC1. is primarily expressed in mucosal surfaces, including the oral cavity, respiratory tract, and upper aerodigestive tract. It plays a role in innate immunity, contributing to host defense against pathogens through mechanisms such as binding to bacterial lipopolysaccharides (LPS), modulating inflammation, and maintaining epithelial barrier integrity.

The antibody is typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant Lplunc1 protein. It is utilized in applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate Lplunc1's expression patterns, localization, and regulation in tissues. Studies using this antibody have highlighted Lplunc1's involvement in chronic inflammatory conditions (e.g., cystic fibrosis, chronic rhinosinusitis), airway infections, and cancers, where its expression is often dysregulated. Researchers also employ it to explore Lplunc1's structural domains—an N-terminal hydrophobic region for lipid interaction and a C-terminal globular domain for immune signaling. Its role in mucosal immunity and potential as a biomarker or therapeutic target underscores the antibody's relevance in both basic and translational research.