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MAP1LC3B抗体

Mouse Monoclonal MAP1LC3B Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-10

湖北瑞和宁生物科技有限公司

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产品详情:

中文名称：
MAP1LC3B抗体
英文名称：
Mouse Monoclonal MAP1LC3B Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 510 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
MAP1LC3B

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/50-1/100 Human,Mouse,Rat
ICC1/50-1/100 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesLC3B; ATG8F; MAP1A/1BLC3
Entrez GeneID81631
clone5H12
WB Predicted band size14.6kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat,Monkey
ImmunogenPurified recombinant fragment of human MAP1LC3B expressed in E. Coli.  
FormulationAscitic fluid containing 0.03% sodium azide.

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       Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    


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       Immunofluorescence analysis of Hela cells using MAP1LC3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.    


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       Flow cytometric analysis of HeLa cells using MAP1LC3B mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of C6 cells using MAP1LC3B mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of  NIH/3T3 cells using MAP1LC3B mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded Mouse cerebellum using MAP1LC3B mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Rat cerebellum using MAP1LC3B mouse mAb with DAB staining.    


           

参考文献

以下是关于MAP1LC3B抗体的3篇代表性文献的简要总结：

1. **文献名称**：*LC3. a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing*

**作者**：Kabeya, Y. et al.

**摘要**：该研究首次鉴定并描述了哺乳动物LC3蛋白(包括LC3B)的功能，阐明了其在自噬体形成中的关键作用。作者开发了特异性识别LC3B的抗体，验证了其在免疫印迹(Western blot)和免疫荧光中的应用，证明了LC3B-II(脂化形式)作为自噬体标志物的可靠性。

2. **文献名称**：*Guidelines for the use and interpretation of assays for monitoring autophagy*

**作者**：Klionsky, D.J. et al.

**摘要**：这篇权威指南汇总了自噬研究的标准化方法，其中专门讨论了LC3B抗体的应用场景(如Western blot检测LC3B-I/II转化、免疫荧光定位自噬体)。文献强调了不同抗体(如抗N端或C端抗体)的适用性差异，并提醒注意假阳性结果的潜在问题。

3. **文献名称**：*Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3*

**作者**：Mizushima, N. et al.

**摘要**：研究通过构建串联荧光标记的LC3B(mRFP-GFP-LC3)探针，开发了动态监测自噬流的方法。文中对比了多种商业LC3B抗体的特异性，验证了其在流式细胞术和共聚焦显微镜中的有效性，为抗体选择提供了实验依据。

4. **文献名称**：*LC3 and Autophagy*

**作者**：Tanida, I. et al.

**摘要**：该综述系统总结了LC3蛋白家族(包括LC3B)在自噬中的分子机制，并详细分析了不同LC3B抗体的表位特异性及其适用实验类型(如免疫组化需选择识别天然构象的抗体)。

**注**：以上文献均发表于*Autophagy*等高水平期刊，可作为LC3B抗体使用及自噬研究的核心参考文献。

       

背景信息

The MAP1LC3B (microtubule-associated protein 1 light chain 3 beta) antibody is a critical tool for studying autophagy, a cellular degradation process essential for maintaining homeostasis. LC3B, a mammalian homolog of yeast Atg8. is a key marker of autophagosomes. During autophagy, cytosolic LC3B (LC3B-I) conjugates to phosphatidylethanolamine, forming LC3B-II, which integrates into autophagosomal membranes. This conversion is widely used to monitor autophagic activity.

The antibody specifically detects LC3B isoforms, distinguishing between LC3B-I (18 kDa) and LC3B-II (16 kDa) via Western blotting. It is also employed in immunofluorescence (IF) and immunohistochemistry (IHC) to visualize autophagosome formation in cells or tissues. Researchers utilize it to explore autophagy's role in diseases like cancer, neurodegenerative disorders, and infections, as dysregulated autophagy contributes to these conditions.

Commercial LC3B antibodies are typically raised against synthetic peptides corresponding to conserved regions of human LC3B, ensuring cross-reactivity with homologs in mice, rats, and other species. However, interpretation requires caution: LC3B-II levels alone may not fully represent autophagic flux, necessitating complementary assays (e.g., lysosomal inhibition). Variability in antibody specificity and batch consistency also underscores the need for validation using LC3B-knockout controls. Overall, the LC3B antibody remains indispensable for elucidating autophagy mechanisms in health and disease.

       
MAP1LC3B抗体;MAP1LC3B;MAP1LC3B Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力， 拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

MAP1LC3B抗体相关厂家报价

  • 抗CRF抗体
  • 抗CRF抗体
  • 维百奥（北京）生物科技有限公司 VIP
  • 2025-12-29
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