FOS抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using FOS mouse mAb against HeLa (1), and HeLa (2) cell lysate.    

  
  

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     Flow cytometric analysis of HeLa cells using FOS mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using FOS mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using FOS mouse mAb with DAB staining.    

  
  

FOS antibodies are essential tools in molecular and cellular biology research, targeting members of the FOS protein family, which includes c-Fos, FosB, Fra-1. and Fra-2. These proteins are transcription factors that dimerize with JUN family proteins to form the AP-1 (Activator Protein-1) complex, a key regulator of gene expression in processes like cell proliferation, differentiation, apoptosis, and stress responses. c-Fos, the most studied member, is an immediate-early gene product rapidly induced by growth factors, cytokines, or neuronal activity, making it a marker for cellular activation. Dysregulation of FOS proteins is linked to cancer, immune disorders, and neurodegenerative diseases.

FOS antibodies enable the detection, localization, and quantification of FOS proteins in experimental models. They are widely used in techniques such as Western blotting, immunohistochemistry, immunofluorescence, and chromatin immunoprecipitation (ChIP). For instance, in neuroscience, c-Fos antibodies help map neuronal activation in response to stimuli, while in cancer research, they assess AP-1 activity in tumor progression. Challenges include distinguishing between FOS family members due to structural similarities and ensuring specificity across species. High-quality, validated antibodies are critical for reliable data, particularly given the transient expression and low basal levels of FOS proteins. Their application continues to advance understanding of cellular signaling pathways and disease mechanisms.