FOXO1抗体

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using FOXO1 mouse mAb against Hela (1), HEK293 (2), MCF-7(3), and C6 (4) cell lysate.    

  
  

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     Immunofluorescence analysis of Hela cells using FOXO1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.    

  
  

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     Flow cytometric analysis of C6 cells using FOXO1 mouse mAb (green) and negative control (red).    

  
  

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     Flow cytometric analysis of NIH/3T3 cells using FOXO1 mouse mAb (green) and negative control (red).    

  
  

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     Flow cytometric analysis of COS7 cells using FOXO1 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human intima cancer tissues using FOXO1 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Mouse cerebellum using FOXO1 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Rat brain(A)Rat kidney(B) using FOXO1 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Rabbit spinal cord(A)Rabbit kidney(B) using FOXO1 mouse mAb with DAB staining.    

  
  

The FOXO1 antibody is a crucial tool in studying the Forkhead box O1 (FOXO1) protein, a member of the evolutionarily conserved FOXO transcription factor family. FOXO1 plays a pivotal role in regulating cellular processes such as apoptosis, autophagy, cell cycle arrest, and stress resistance. It acts as a downstream effector of the insulin/PI3K/AKT signaling pathway, where AKT-mediated phosphorylation triggers its cytoplasmic sequestration and inactivation. Dysregulation of FOXO1 is implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders.

FOXO1 antibodies are widely used in research to detect protein expression, localization, and post-translational modifications (e.g., phosphorylation at Ser256 or Thr24) through techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help investigate FOXO1's tumor-suppressive roles in certain cancers or its context-dependent oncogenic functions. In metabolic studies, they aid in exploring FOXO1's regulation of gluconeogenesis in the liver and its interaction with PPARγ in adipocyte differentiation.

Commercial FOXO1 antibodies are typically raised against specific epitopes, with validation in knockout controls being critical due to potential cross-reactivity with other FOXO family members (FOXO3. FOXO4. FOXO6). Their applications span basic research, drug development, and diagnostic investigations into diseases linked to insulin signaling and oxidative stress pathways.