HFE抗体

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     Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    

  
  

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     Immunofluorescence analysis of HepG2 cells using HFE mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.    

  
  

The HFE antibody is primarily associated with research on hereditary hemochromatosis (HH), a genetic disorder characterized by excessive iron absorption. The HFE gene encodes a protein involved in regulating iron homeostasis by interacting with transferrin receptor 1 (TfR1) and modulating hepcidin expression, a key hormone controlling iron balance. Mutations in HFE, particularly C282Y and H63D, disrupt this regulation, leading to iron overload and organ damage.

HFE antibodies are tools used to detect and study the HFE protein in experimental settings, such as Western blotting, immunohistochemistry, or ELISA. They help elucidate HFE's expression patterns, interaction partners, and pathological mechanisms in HH. While genetic testing remains the gold standard for HH diagnosis, HFE antibodies contribute to understanding protein dysfunction in cellular models or tissue samples.

Research using these antibodies has advanced insights into iron metabolism disorders and potential therapeutic targets. However, their clinical diagnostic utility is limited, as most HH cases are identified via genetic screening. Current studies focus on HFE's role beyond iron regulation, including immune modulation and associations with diseases like cancer or neurodegeneration. Challenges include antibody specificity and standardization across assays, underscoring the need for continued refinement in HH-related research tools.