AIF抗体

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     Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    

  
  

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     Western blot analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.    

  
  

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     Western blot analysis using AIF mouse mAb against CHO3D10(1),COS7(2),F9(3),L1210(4),C6(5),C2C12(6),NIH/3T3(7),Raw264.7(8),PC-12(9) cell lysate.    

  
  

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     Immunofluorescence analysis of NIH/3T3 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    

  
  

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     Immunofluorescence analysis of RSC-96 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.    

  
  

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     Immunofluorescence analysis of NIH3T3 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.    

  
  

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     Flow cytometric analysis of HepG2 cells using AIF mouse mAb (blue) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using AIF mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using AIF mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using AIF mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using AIF mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Mouse liver(A) Mouse kidney(B) using AIF mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Rat liver(A) Rat kidney(B) using AIF mouse mAb with DAB staining.    

  
  

**Background of AIF Antibody**

The Apoptosis-Inducing Factor (AIF) is a mitochondrial flavoprotein with dual roles in cellular homeostasis and apoptosis. Initially identified for its involvement in caspase-independent programmed cell death, AIF translocates from mitochondria to the nucleus under apoptotic stimuli, triggering chromatin condensation and DNA fragmentation. Beyond apoptosis, AIF is critical for maintaining mitochondrial structure, bioenergetics, and redox balance, supported by its role in regulating respiratory chain complexes.

AIF antibodies are essential tools in studying these processes. They enable detection of AIF’s subcellular localization (e.g., via immunofluorescence) and expression levels (e.g., via Western blot), helping to dissect its pro-apoptotic versus pro-survival functions. Research using AIF antibodies has revealed its involvement in neurodegenerative diseases (e.g., Parkinson’s, Alzheimer’s), ischemic injuries, and cancer, where AIF dysregulation correlates with pathological outcomes.

Structurally, AIF antibodies often target conserved epitopes, such as the N-terminal mitochondrial localization signal or C-terminal DNA-binding domain. Some antibodies distinguish between full-length AIF (∼67 kDa) and its truncated forms, which arise during apoptosis. The development of selective AIF antibodies has advanced therapeutic exploration, including strategies to inhibit AIF-mediated apoptosis in neurodegeneration or enhance its pro-death role in cancer. Overall, AIF antibodies remain pivotal in unraveling the multifaceted biology of AIF and its translational potential.