PINCH抗体 新品

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     Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    

  
  

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     Western blot analysis using PINCH mouse mAb against A549 (1), Jurkat (2), and Hela (3) cell lysate.    

  
  

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     Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    

  
  

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     Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and negative control (red).    

  
  

PINCH (Particularly Interesting New Cysteine-Histidine-rich protein) antibodies are immunological tools targeting PINCH proteins, a family of adaptor molecules critical for cell-matrix adhesion and signaling. PINCH proteins, primarily PINCH-1 and PINCH-2. contain five LIM domains that mediate protein-protein interactions, enabling their role in bridging integrin-linked kinase (ILK) to other focal adhesion components. These proteins localize to focal adhesions, dynamic structures linking the extracellular matrix (ECM) to the actin cytoskeleton, and regulate cell migration, proliferation, and survival through pathways like Ras/MAPK and PI3K/AKT.

PINCH proteins are essential in development and tissue homeostasis, with dysregulation linked to pathologies such as cancer, fibrosis, and cardiovascular diseases. Overexpression of PINCH-1. for instance, correlates with tumor progression and poor prognosis in various cancers by enhancing ECM remodeling and metastatic potential. PINCH-2 often compensates for PINCH-1 loss but exhibits distinct tissue-specific roles.

PINCH antibodies are widely used in research to study focal adhesion dynamics, protein interaction networks, and disease mechanisms. They enable techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess PINCH expression, localization, and interactions. Additionally, these antibodies have potential diagnostic applications, as PINCH levels in tissues or biofluids may serve as biomarkers for disease progression or therapeutic response. Their development and optimization continue to advance understanding of cell-ECM communication in health and disease.