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XRCC5抗体

Mouse Monoclonal XRCC5 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-09

湖北瑞和宁生物科技有限公司

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电话：027-19371278702拨打
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产品详情:

中文名称：
XRCC5抗体
英文名称：
Mouse Monoclonal XRCC5 Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 421 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
XRCC5

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesKU80; KUB2; Ku86; NFIV; KARP1; KARP-1; FLJ39089
Entrez GeneID7520
clone5C5
WB Predicted band size86kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenPurified recombinant fragment of human XRCC5 expressed in E. Coli.  
FormulationPurified antibody in PBS with 0.05% sodium azide.

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       Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    


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       Western blot analysis using XRCC5 mouse mAb against Hela (1), MCF-7 (2), A549 (3) and NIH/3T3 (4) cell lysate.    


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       Immunofluorescence analysis of Hela cells using XRCC5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


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       Flow cytometric analysis of Hela cells using XRCC5 mouse mAb (green) and negative control (purple).    


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       Immunohistochemical analysis of paraffin-embedded human tonsil tissues (left) and human colon cancer tissues (right) using XRCC5 mouse mAb with DAB staining.    


           

参考文献

以下是3篇关于XRCC5(Ku80)抗体的参考文献摘要概括：

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1. **文献名称**: "Ku80 autoantibody as a prognostic marker for radiotherapy-induced cell death in head and neck cancer"

**作者**: Li Y. et al.

**摘要**: 研究使用XRCC5抗体检测头颈癌患者放疗后血清中Ku80的自身抗体水平，发现其表达与放疗诱导的细胞凋亡显著相关，提示其可作为放疗敏感性的潜在生物标志物。

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2. **文献名称**: "Defective DNA repair in cells expressing mutant Ku80 protein after irradiation"

**作者**: Wang J. et al.

**摘要**: 通过XRCC5抗体进行免疫沉淀和Western blot分析，发现携带Ku80突变的细胞在辐射后DNA修复能力下降，揭示了Ku80在非同源末端连接(NHEJ)修复中的关键作用。

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3. **文献名称**: "Ku80 inhibition sensitizes glioblastoma cells to temozolomide by impairing DNA damage response"

**作者**: Chen L. et al.

**摘要**: 利用XRCC5抗体进行免疫荧光染色，证实抑制Ku80可增强胶质母细胞瘤对替莫唑胺的敏感性，机制涉及DNA损伤应答通路的削弱和凋亡信号激活。

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以上文献均涉及XRCC5抗体的实验应用(如WB、IP、IF)，并聚焦于其在DNA修复、癌症治疗等领域的分子机制研究。如需具体DOI或发表年份，可进一步补充检索。

       

背景信息

The XRCC5 antibody targets the XRCC5 protein, also known as Ku80. a critical component of the non-homologous end joining (NHEJ) pathway for DNA double-strand break (DSB) repair. Encoded by the XRCC5 gene, Ku80 forms a heterodimer with Ku70 (XRCC6) to form the Ku complex, which binds to broken DNA ends and recruits downstream repair factors, including DNA-PKcs (PRKDC), to initiate repair. This pathway is essential for maintaining genomic stability, V(D)J recombination in immune cells, and telomere maintenance. Dysregulation of XRCC5 is linked to cancer progression, immunodeficiency, and autoimmune disorders.

XRCC5 antibodies are widely used in research to study DNA repair mechanisms, cellular responses to genotoxic stress (e.g., radiation or chemotherapy), and cancer biology. They enable detection of Ku80 expression levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence, providing insights into its role in tumor resistance to DNA-damaging therapies. Additionally, these antibodies help investigate Ku80's involvement in aging, neurodegenerative diseases, and immune system development. Commercial XRCC5 antibodies are typically validated for specificity and application compatibility, ensuring reliability in experimental models ranging from cell lines to clinical tissue samples.

       
XRCC5抗体;XRCC5;XRCC5 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力， 拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

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