HSP27抗体

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     Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    

  
  

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     Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jurkat (3), A431 (4), HEK293(5), HepG2 (6) and PC-12 (7) cell lysate.    

  
  

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     Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    

  
  

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     Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and negative control (purple).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human brain tissues (left) and esophageal cancer tissues (right) using HSP27 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and cardiac muscle tissues (right) using HSP27 mouse mAb with DAB staining.    

  
  

Heat shock protein 27 (HSP27), also known as HSPB1. is a member of the small heat shock protein (sHSP) family. It is a 27 kDa molecular chaperone ubiquitously expressed in eukaryotic cells. HSP27 plays a critical role in cellular stress responses, particularly under conditions like heat shock, oxidative stress, or chemical toxicity. It stabilizes protein folding, prevents aggregation of denatured proteins, and supports cellular survival by inhibiting apoptosis through interactions with key signaling pathways, including the AKT and MAPK pathways. Structurally, HSP27 forms dynamic oligomers, and its function is regulated by phosphorylation, which modulates its chaperone activity and cellular localization.

HSP27 antibodies are essential tools for studying its expression, localization, and functional roles in both physiological and pathological contexts. These antibodies enable detection of HSP27 in techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Research has highlighted HSP27's dual role in cancer—acting as a tumor promoter by enhancing cell survival, metastasis, and therapy resistance, while also exhibiting tumor-suppressive effects in certain contexts. Elevated HSP27 levels are associated with neurodegenerative diseases (e.g., Alzheimer’s), inflammatory disorders, and cardiovascular conditions, making it a biomarker of interest.

The development of HSP27 antibodies has advanced understanding of its therapeutic potential, including targeting HSP27 in cancer treatment or leveraging its protective roles in degenerative diseases. However, its context-dependent functions necessitate careful interpretation of experimental results using validated antibodies to ensure specificity.