CREB1抗体

CREB1 (cAMP Response Element-Binding Protein 1) is a critical transcription factor involved in regulating gene expression in response to various cellular signals, including cAMP, calcium, and growth factors. It belongs to the leucine zipper family of DNA-binding proteins and activates target genes by binding to cAMP response elements (CREs) in their promoter regions. CREB1 plays essential roles in processes such as neuronal plasticity, memory formation, circadian rhythm regulation, and cell survival. Antibodies targeting CREB1 are widely used in research to detect its expression, phosphorylation status (e.g., at Ser133. a key modification for its activation), and subcellular localization. These antibodies enable techniques like Western blotting, immunohistochemistry, immunofluorescence, and chromatin immunoprecipitation (ChIP), aiding in studies of CREB1's involvement in signaling pathways (e.g., cAMP/PKA, MAPK) and its dysregulation in diseases like cancer, neurodegenerative disorders, and metabolic syndromes. Specificity varies among antibodies, with some recognizing total CREB1 and others targeting phosphorylated or isoform-specific forms. Validation via knockout controls or peptide blocking is critical to ensure reliability.