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HIF1A抗体

Mouse Monoclonal HIF1A Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-09

湖北瑞和宁生物科技有限公司

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联系人：周经理
电话：027-19371278702拨打
手机：18008634902 拨打
邮箱：19384116026@163.com

产品详情:

中文名称：
HIF1A抗体
英文名称：
Mouse Monoclonal HIF1A Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 393 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
HIF1A

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Monkey
IF咨询技术 Human,Mouse,Monkey
IHC1/200 - 1/1000 Human,Mouse,Monkey
ICC1/200 - 1/1000 Human,Mouse,Monkey
FCM咨询技术 Human,Mouse,Monkey
Elisa1/10000 Human,Mouse,Monkey
   

产品详情

AliasesHIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF1A
Entrez GeneID3091
clone1A3
WB Predicted band size120kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Monkey
ImmunogenPurified recombinant fragment of human HIF1A expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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       Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    


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       Western blot analysis using HIF1A mouse mAb against Cos7 (1), Hela (2), Jurkat (3), RAJI (4) and NIH/3T3 (5) cell lysate.    


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       Immunofluorescence analysis of Hela cells using HIF1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


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       Immunohistochemical analysis of paraffin-embedded human liver cancer tissues (left) and lung cancer tissues (right) using HIF1A mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues (left) and brain tumor tissues (right) using HIF1A mouse mAb with DAB staining.    


           

参考文献

以下是3篇关于HIF1A抗体的代表性文献摘要：

1. **"Hypoxia-inducible factor 1α is a new marker of aggressive serous ovarian cancer"**

- Authors: Birner P. et al. (2011)

- 摘要：该研究使用HIF1A抗体(克隆H1alpha67)通过免疫组化检测卵巢癌组织，发现HIF1α高表达与肿瘤侵袭性和不良预后显著相关，验证了该抗体在临床样本中的特异性。

2. **"Comparison of commercial antibodies for detection of HIF-1α in human cancer cells"**

- Authors: Vordermark D. et al. (2005)

- 摘要：系统性比较了多种市售HIF1A抗体(包括BD Biosciences和Novus Biologicals产品)在Western blot和免疫荧光中的表现，提出低氧条件(1% O₂)下抗体检测敏感性优化的关键条件。

3. **"Regulation of hypoxia-inducible factor 1α by the ubiquitin-proteasome pathway"**

- Authors: Salceda S. & Caro J. (1997)

- 摘要：早期经典研究，利用HIF1A抗体(自制多克隆抗体)证实HIF-1α在常氧条件下通过泛素-蛋白酶体途径降解，奠定后续抗体功能验证的基础。

注：如需具体DOI或期刊名称可进一步补充。建议优先选择近5年文献并结合实验类型(如ChIP、IF等)筛选抗体适用性。

       

背景信息

HIF1A (hypoxia-inducible factor 1-alpha) is a critical transcriptional regulator that enables cellular adaptation to low oxygen conditions. As the oxygen-sensitive subunit of the HIF1 heterodimer, it partners with HIF1β (ARNT) to activate genes involved in angiogenesis, glycolysis, proliferation, and survival under hypoxia. Dysregulation of HIF1A is implicated in cancer progression, cardiovascular diseases, and ischemic disorders, making it a key research target in pathophysiology and therapeutic development.

HIF1A antibodies are essential tools for detecting and quantifying HIF1A protein expression, localization, and activation in experimental models. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to study hypoxia-responsive pathways. Specific antibodies can distinguish between normoxic degradation (mediated by prolyl hydroxylases and VHL ubiquitination) and hypoxic stabilization of HIF1A, aiding mechanistic studies. Some antibodies target post-translational modifications (e.g., hydroxylation) or isoforms (HIF1A vs. HIF2A/EPAS1), enabling precise functional analyses.

In cancer research, HIF1A antibodies help assess tumor hypoxia, metastatic potential, and therapeutic resistance. However, variability in antibody specificity across commercial sources requires careful validation using knockout controls. Despite challenges, these reagents remain indispensable for unraveling HIF1A's role in health and disease.

       
HIF1A抗体;HIF1A;HIF1A Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力， 拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

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