ERK3抗体

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     Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    

  
  

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     Flow cytometric analysis of Hela cells using ERK3 mouse mAb (green) and negative control (purple).    

  
  

**Background of ERK3 Antibody**

Extracellular signal-regulated kinase 3 (ERK3), also known as MAPK6. is a member of the mitogen-activated protein kinase (MAPK) family, which plays critical roles in transducing extracellular signals to intracellular responses. Unlike canonical MAPKs such as ERK1/2. ERK3 exhibits distinct structural and functional characteristics. It contains a unique C-terminal extension and lacks the conserved Thr-X-Tyr activation motif found in other MAPKs, instead harboring a Ser-Glu-Gly (SEG) activation loop. ERK3 is activated through phosphorylation, though its upstream regulators and downstream effectors remain less defined compared to other MAPKs.

ERK3 has been implicated in diverse cellular processes, including cell cycle regulation, differentiation, migration, and stress responses. Studies suggest its involvement in embryonic development, cancer progression, and immune regulation. For example, ERK3 interacts with the MK5/PRAK kinase, influencing cytoskeletal dynamics and cell motility. Its expression is often dysregulated in cancers, highlighting potential therapeutic relevance.

ERK3 antibodies are essential tools for studying its expression, localization, and activation. They enable detection via techniques like Western blotting, immunofluorescence, and immunohistochemistry. However, due to ERK3's homology with ERK4 (MAPK4), antibody specificity must be rigorously validated using knockout controls. Research on ERK3 is ongoing to clarify its signaling mechanisms and physiological roles, making reliable antibodies critical for advancing understanding in cellular and disease contexts.