EEF2抗体

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     Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    

  
  

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     Western blot analysis using EEF2 mouse mAb against HepG2 (1), Hela (2), HEK293 (3) and A431 (4) cell lysate.    

  
  

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     Immunofluorescence analysis of 3T3-L1 cells using EEF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human liver cancer tissues (left) and kidney cancer tissues (right) using EEF2 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues (left) and tonsil tissues (right) using EEF2 mouse mAb with DAB staining.    

  
  

**Background of EEF2 Antibodies**

Eukaryotic Elongation Factor 2 (EEF2) is a GTP-binding protein critical for protein synthesis, facilitating the translocation of transfer RNA (tRNA) and messenger RNA (mRNA) during the elongation phase of translation. It is a conserved, 96 kDa protein found in all eukaryotes. EEF2 antibodies are immunological tools designed to detect and study this protein’s expression, localization, and function in biological systems.

Research involving EEF2 antibodies often focuses on cellular processes such as translation regulation, stress responses, and apoptosis. For instance, EEF2 activity is modulated by phosphorylation (e.g., at Thr56 by EEF2 kinase), which temporarily halts translation under stress conditions. Dysregulation of EEF2 is linked to diseases, including cancers (e.g., upregulated in certain tumors), neurodegenerative disorders (e.g., Alzheimer’s disease), and infections (e.g., bacterial toxins like Pseudomonas exotoxin A target EEF2).

EEF2 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein levels in tissues or cultured cells. They also help investigate post-translational modifications or interactions with other molecules. Additionally, autoantibodies against EEF2 are studied in autoimmune conditions, highlighting their diagnostic relevance.

In summary, EEF2 antibodies serve as essential reagents for probing translational control mechanisms and disease pathologies, bridging molecular biology with clinical research.