BECN1抗体

+ +

•  

   

     Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    

  
  

•  

   

     Western blot analysis using BECN1 mouse mAb against Hela (1), A431 (2), MCF-7 (3), RAJI (4), Jurkat (5) and SKBR-3 (6) cell lysate.    

  
  

•  

   

     Flow cytometric analysis of RAJI cells using BECN1 mouse mAb (green) and negative control (purple).    

  
  

•  

   

     Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and liver cancer tissues (right) using BECN1 mouse mAb with DAB staining.    

  
  

The BECN1 antibody targets Beclin-1. a 60 kDa protein encoded by the *BECN1* gene, which plays a central role in autophagy regulation. Beclin-1 is a key component of the class III phosphatidylinositol 3-kinase (PI3K-III) complex, essential for autophagosome formation and vesicle trafficking. It interacts with proteins like UVRAG, ATG14L, and BCL-2. balancing autophagy induction and apoptosis. Dysregulation of Beclin-1 is linked to cancer, neurodegenerative disorders (e.g., Alzheimer’s), and infections, as autophagy modulates cellular homeostasis and pathogen clearance. In cancer, reduced Beclin-1 expression correlates with tumor progression, highlighting its tumor-suppressive role.

BECN1 antibodies are widely used in research to study autophagy mechanisms, protein-protein interactions, and disease pathways. Common applications include Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect Beclin-1 expression levels, localization, or post-translational modifications. Commercial antibodies are often validated for specificity in *BECN1* knockout/knockdown models. Researchers also employ these antibodies to explore therapeutic strategies targeting autophagy, such as cancer treatment or neuroprotection. Variants include monoclonal (e.g., rabbit or mouse origin) and polyclonal antibodies, with selection depending on experimental needs. Proper controls are critical due to potential cross-reactivity with homologous proteins. Overall, BECN1 antibodies are indispensable tools for understanding autophagy-related physiology and pathology.