NR3C1抗体

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     Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    

  
  

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     Western blot analysis using NR3C1 mouse mAb against Hela cell lysate.    

  
  

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     Immunofluorescence analysis of PC-2 cells using NR3C1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    

  
  

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     Flow cytometric analysis of K562 cells using NR3C1 mouse mAb (green) and negative control (purple).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using NR3C1 mouse mAb with DAB staining.    

  
  

**Background of NR3C1 Antibody**

The NR3C1 (Nuclear Receptor Subfamily 3 Group C Member 1) gene encodes the glucocorticoid receptor (GR), a ligand-activated transcription factor critical for mediating the effects of glucocorticoids, which regulate diverse physiological processes such as immune response, metabolism, and stress adaptation. The GR protein consists of functional domains, including a ligand-binding domain and a DNA-binding domain, enabling it to translocate to the nucleus upon glucocorticoid binding, modulate gene expression, and interact with other signaling pathways.

NR3C1 antibodies are essential tools for studying GR expression, localization, and function in research. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect GR levels in tissues or cells, assess its activation status, or investigate dysregulation in diseases such as cancer, metabolic disorders, and neuropsychiatric conditions. Specific NR3C1 antibodies target distinct epitopes (e.g., N-terminal, C-terminal) to study full-length GR isoforms or splice variants. Their applications extend to understanding glucocorticoid resistance mechanisms, hormone signaling crosstalk, and therapeutic responses in conditions treated with glucocorticoids. Validated antibodies ensure specificity, minimizing cross-reactivity with related nuclear receptors, and are crucial for reliable experimental outcomes.