基本信息 产品详情 公司简介 推荐产品
  • 首页
  • 个人中心
  • 委托采购
  • 采购清单
网站主页 化工产品目录 生物化工 抗体 4-OCT抗体 4-Oct抗体
  • 4-Oct抗体
  • 4-Oct抗体
  • 4-Oct抗体

1/3

4-Oct抗体

Mouse Monoclonal 4-Oct Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-09

湖北瑞和宁生物科技有限公司

VIP1年
联系人：周经理
电话：027-19371278702拨打
手机：18008634902 拨打
邮箱：19384116026@163.com

产品详情:

中文名称：
4-Oct抗体
英文名称：
Mouse Monoclonal 4-Oct Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 340 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
4-Oct

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC咨询技术 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesOCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4; MGC22487; POU5F1
Entrez GeneID5460
clone7E7
WB Predicted band size45kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenSynthesized peptide derived from internal of human Oct4.
FormulationAscitic fluid containing 0.03% sodium azide.

+ +

  •  

     

       Western blot analysis using Oct4 mouse mAb against NTERA-2 (1) cell lysate.    


  •  

     

       Immunofluorescence analysis of NTERA-2 cells using Oct4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


  •  

     

       Flow cytometric analysis of Jurkat cells using Oct4 mouse mAb (green) and negative control (purple).    


           

参考文献

以下是关于Oct-4抗体的3篇参考文献及其摘要概括：

---

1. **文献名称**: *The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells*

**作者**: Mitsui, K. et al.

**摘要**: 本研究揭示了Oct-4与Nanog蛋白协同维持胚胎干细胞(ES细胞)多能性的机制。通过Oct-4抗体的免疫沉淀实验，发现Oct-4直接调控Nanog的表达，二者共同抑制分化相关基因，是干细胞自我更新的关键转录因子。

2. **文献名称**: *Germ cell tumors express the embryonic pluripotency marker OCT4/POU5F1*

**作者**: Jones, T.D. et al.

**摘要**: 文章利用Oct-4抗体对生殖细胞肿瘤(如精原细胞瘤)进行免疫组化分析，发现Oct-4蛋白在肿瘤细胞中高表达，提示其可作为诊断标记物，并为生殖细胞肿瘤的干细胞起源理论提供依据。

3. **文献名称**: *Quantitative expression of Oct-4 defines differentiation, dedifferentiation or self-renewal of ES cells*

**作者**: Niwa, H. et al.

**摘要**: 通过Oct-4抗体的定量检测，证明Oct-4蛋白表达水平对胚胎干细胞的命运起决定性作用：过高或过低分别导致自发分化或去分化，强调其在多能性维持中的精确调控作用。

---

**备注**：Oct-4(POU5F1)是干细胞与发育生物学领域的核心蛋白，其抗体广泛应用于多能性研究及癌症标志物检测。上述文献涵盖分子机制、诊断应用及功能验证，代表该抗体的关键研究方向。

       

背景信息

The 4-Oct antibody, commonly referred to as anti-Oct-4 antibody, targets the Octamer-binding transcription factor 4 (Oct-4), a pivotal protein encoded by the *POU5F1* gene in humans. Oct-4 is a member of the POU-domain transcription factor family and plays a critical role in maintaining pluripotency and self-renewal in embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). It regulates the expression of genes essential for early embryonic development and cellular reprogramming.

Antibodies against Oct-4 are widely used in stem cell research, cancer biology, and regenerative medicine to identify and characterize pluripotent cells. In cancer studies, Oct-4 expression is linked to germ cell tumors and某些 cancers with stem-like properties, making it a potential diagnostic or prognostic marker. However, specificity challenges exist due to splice variants (e.g., Oct-4A vs. Oct-4B) and pseudogenes, necessitating validation via controls like knockout cell lines.

These antibodies are employed in techniques like immunohistochemistry, flow cytometry, and Western blot. Their applications extend to quality control in iPSC generation and studying mechanisms of cellular differentiation. Despite their utility, variations in antibody clones (e.g., C30A3. 9E2) may yield differing results, emphasizing the need for context-specific validation. Overall, Oct-4 antibodies remain indispensable tools for exploring pluripotency and disease mechanisms.

       
4-Oct抗体;4-Oct;4-Oct Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力， 拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

4-Oct抗体相关厂家报价

内容声明
official 进入官方账号
拨打电话 立即询价