CCNB1抗体

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     Western blot analysis using CCNB1 mouse mAb against Hela (1), Jurkat (2), K562 (3) and PC-12 (4) cell lysate.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and ovary tumour tissues (right) using CCNB1 mouse mAb with DAB staining.    

  
  

The CCNB1 antibody targets Cyclin B1. a key regulatory protein encoded by the *CCNB1* gene, which plays a critical role in cell cycle progression. Cyclin B1 partners with cyclin-dependent kinase 1 (CDK1) to form the maturation-promoting factor (MPF), essential for the G2/M phase transition. This complex drives mitotic entry by phosphorylating substrates involved in nuclear envelope breakdown, chromatin condensation, and spindle assembly. Cyclin B1 expression peaks during late G2 and degrades via the ubiquitin-proteasome system during metaphase-anaphase transition, ensuring proper cell cycle control.

CCNB1 antibodies are widely used in research to study cell cycle dynamics, particularly in cancer biology. Overexpression of Cyclin B1 is linked to uncontrolled proliferation, genomic instability, and poor prognosis in various cancers. These antibodies enable detection of Cyclin B1 levels through techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), aiding in the assessment of tumor aggressiveness and therapeutic response. Additionally, they help explore Cyclin B1's role in checkpoint regulation, DNA damage responses, and its potential as a biomarker for targeted therapies. Available in monoclonal or polyclonal forms, CCNB1 antibodies are critical tools for understanding cell cycle mechanisms and disease pathology.