CHUK抗体

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     Western blot analysis using CHUK mouse mAb against Raji (1), Jurkat (2) and THP-1 (3) cell lysate.    

  
  

**Background of CHUK Antibody**

The CHUK (conserved helix-loop-helix ubiquitous kinase) antibody targets the CHUK protein, also known as IκB kinase alpha (IKKα), a critical component of the IKK complex within the NF-κB signaling pathway. CHUK/IKKα, along with IKKβ and NEMO (IKKγ), regulates the activation of NF-κB transcription factors by phosphorylating inhibitory IκB proteins, leading to their degradation and subsequent nuclear translocation of NF-κB. This pathway is pivotal in mediating immune responses, inflammation, cell survival, and proliferation.

CHUK antibodies are widely used in research to study the expression, localization, and function of IKKα in various biological contexts. They are essential tools in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to investigate CHUK's role in diseases such as cancer, autoimmune disorders, and chronic inflammation. For example, dysregulation of CHUK/IKKα has been linked to skin malignancies, rheumatoid arthritis, and aberrant immune activation.

These antibodies are typically developed in hosts like rabbits or mice, with specificity validated through knockout controls or siRNA-based silencing. Researchers rely on CHUK antibodies to dissect signaling mechanisms, assess phosphorylation states, and explore therapeutic targets. Understanding CHUK's dual roles—canonical (NF-κB activation) and non-canonical (e.g., epidermal differentiation)—highlights its complexity, making specific antibodies crucial for unraveling its diverse cellular functions.